EphB2 / Hek5 recombinant protein

Recombinant Human EphB2 / Hek5 Protein

Cat. Num. AAA2546095

• Gene Names: EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: EphB2 / Hek5; Recombinant Human EphB2 / Hek5 Protein; EPHB2; CAPB; DRT; EPHT3; ERK; Hek5; PCBC; Tyro5; EphB2 / Hek5 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
• Sequence Length: 986

• Application Notes: The recombinant human EPHB2 consists of 532 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 66 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for EphB2 / Hek5 recombinant protein

Background: Ephrin type -B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Description: A DNA sequence encoding the human EPHB2 (NP_059145.2) (Met 1-Leu 543) was expressed with six amino acids (ENLYFQ) at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 111118978
• NCBI GeneID: 2048
• NCBI Accession #: NP_059145.2
• NCBI GenBank Nucleotide #: NM_017449.4
• UniProt Accession #: P29323; O43477; Q5T0U6; Q5T0U7; Q5T0U8
• Molecular Weight: 110,030 Da
• NCBI Official Full Name: ephrin type-B receptor 2 isoform 1
• NCBI Official Synonym Full Names: EPH receptor B2
• NCBI Official Symbol: EPHB2
• NCBI Official Synonym Symbols: DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5
• NCBI Protein Information: ephrin type-B receptor 2
• UniProt Protein Name: Ephrin type-B receptor 2
• UniProt Gene Name: EPHB2
• UniProt Synonym Gene Names: DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; EK5; hEK5

NCBI Description

This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

EphB2: a receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members. Activated EphB2 recruits RasGAP, down-regulating the Ras-Erk signaling axis and neurite retraction. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB2 interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Point mutations seen in prostate cancer. Overexpressed and required for migration of glioblastoma. Overexpressed and correlated with poor survival in breast cancer. Overexpression and loss of heterozygosity seen in colorectal cancers. Target for immunoconjugate drug therapy .Three splice-variant isoforms have been described.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tumor suppressor

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: axon; cytosol; dendrite; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; transmembrane-ephrin receptor activity

Biological Process: angiogenesis; axon guidance; axonal fasciculation; corpus callosum development; ephrin receptor signaling pathway; inner ear morphogenesis; nervous system development; palate development; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of synaptogenesis; regulation of body fluid levels; urogenital system development

Disease: Prostate Cancer/brain Cancer Susceptibility