Mouse Protein Phosphatase 2AC, Serine, Threonine Monoclonal Antibody | anti-PRKCD antibody

Protein Phosphatase 2AC, Serine, Threonine (PP2AC Ser/Thr)

Cat. Num. AAA603291

• Gene Names: PRKCD; MAY1; PKCD; nPKC-delta
• Reactivity: Chicken, Drosophila, Human, Mouse, Porcine, Rabbit, Rat
• Applications: Western Blot
• Purity: Ascites; Ascites
• Synonyms: Protein Phosphatase 2AC; Serine; Threonine; Monoclonal Antibody; Threonine (PP2AC Ser/Thr); Anti -Protein Phosphatase 2AC; anti-PRKCD antibody

• Host: Mouse
• Reactivity: Chicken, Drosophila, Human, Mouse, Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Clone Number: 0.T.159
• Specificity: Recognizes the catalytic subunit of human PP2A at 36kD. Species Crossreactivity: Mouse and yeast. Predicted to crossreact with rat, porcine, rabbit, chicken and Drosophila.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid, ascites, 0.05% sodium azide, 30% glycerol.

• Applicable Applications for anti-PRKCD antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000. Detected the C-subunit of PP2A in RIPA lysates from A431 cells.; Optimal dilutions to be determined by researcher.
• Immunogen: Synthetic peptide corresponding to amino acids 302- 309 (C-bA- RRTPDYFL) of human PP2A catalytic subunit (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: A431 cell lysate was probed with PP2A, C-subunit (1:1000 dilution). Arrow indicates PP2A, C-subunit (36kD).)

Product Categories/Family for anti-PRKCD antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 47157325
• NCBI GeneID: 5580
• NCBI Accession #: NP_997704.1
• NCBI GenBank Nucleotide #: NM_212539.1
• UniProt Accession #: Q05655; Q15144; Q86XJ6; B0KZ81; B2R834
• Molecular Weight: 77,505 Da
• NCBI Official Full Name: protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: PRKCD
• NCBI Official Synonym Symbols: MAY1; PKCD; nPKC-delta
• NCBI Protein Information: protein kinase C delta type; protein kinase C delta VIII; tyrosine-protein kinase PRKCD
• UniProt Protein Name: Protein kinase C delta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCD
• UniProt Synonym Gene Names: SDK1
• UniProt Entry Name: KPCD_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCD: an AGC kinase of the PKC family. A novel PKC: Ca2 independent but still regulated by PS, DAG and phorbol esters. Caspase-3 appears to liberate a sphingosine-activated catalytic fragment that has altered regulatory mechanisms and substrate specificities.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; Tumor suppressor; Nuclear receptor co-regulator; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intercellular junction; cytosol; nucleus

Molecular Function: calcium-independent protein kinase C activity; protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; insulin receptor substrate binding; metal ion binding; enzyme activator activity; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: B cell proliferation; negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; interleukin-12 production; nerve growth factor receptor signaling pathway; apoptosis; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of filopodium formation; positive regulation of superoxide release; regulation of receptor activity; negative regulation of protein binding; cell structure disassembly during apoptosis; epidermal growth factor receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; platelet activation; fibroblast growth factor receptor signaling pathway; neutrophil activation; protein stabilization; negative regulation of actin filament polymerization; cytokine and chemokine mediated signaling pathway; peptidyl-threonine phosphorylation; cell cycle; phospholipase C activation; negative regulation of inflammatory response; defense response to bacterium; interleukin-10 production; innate immune response; gene expression; immunoglobulin mediated immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; induction of apoptosis by oxidative stress

Disease: Autoimmune Lymphoproliferative Syndrome, Type Iii