Enolase, Muscle Specific (MSE) Recombinant Protein | MSE recombinant protein

Recombinant Enolase, Muscle Specific (MSE)

Cat. Num. AAA2009163

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Enolase; Muscle Specific (MSE); Recombinant Enolase; MSE recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MSIQKIHARE ILDSRGEPTV EVDLHTAKGH FRAAVPSGAS TGIHEALEPR DGDKKRFLGK GVLKAVEHIN KTIGPALIEK KISVVEQEKI DKVMIEMDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN TELILPVPAF NVINGGSHAG NKLAMQEFMV LPVGAASFHD AMRVGAEVYH SLKGVIKAKY GKDATNVGGE GGFAPNILDN HEALELLKAA IAQAGYTDKV VIGMDVAASE FCRDGRYHLD FKSPPHTKRY ITGEQLGEIY RGFIKDYPVV SIEDPFDQDDWEAWKRFVFH VDIQVVGDDL TVTNPKRIAH GAEQHACNCL LLKVNQIGSV TESIQACKLA QSHGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDKAKFAGRKFRN PKAK
• Sequence Length: 434

• Applicable Applications for MSE recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Chicken (Gallus)
• Expression System: Prokaryotic expression
• Residues: Met1~Lys434 (Accession # P07322) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46048765
• NCBI GeneID: 396016
• NCBI Accession #: NP_990450.1
• NCBI GenBank Nucleotide #: NM_205119.1
• UniProt Accession #: P07322
• Molecular Weight: 79.2kDa
• NCBI Official Full Name: beta-enolase
• NCBI Official Synonym Full Names: enolase
• NCBI Official Symbol: ENO3
• NCBI Protein Information: beta-enolase; phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Beta-enolase
• UniProt Gene Name: ENO3
• UniProt Entry Name: ENOB_CHICK

Uniprot Description

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Subunit structure: Homodimer. Interacts with PNKD

By similarity.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the enolase family.