ENO3 active protein

ENO3, 1-434aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204286

• Gene Names: ENO3; MSE; GSD13
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ENO3; 1-434aa; Human; His tag; E Coli (Bioactivity Validated); Beta-enolase; MSE; ENO3 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK
• Sequence Length: 434

• Applicable Applications for ENO3 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 5,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
• Assay: 1. Prepare 180ul assay buffer into a suitable container: The concentrations are 80 mM Triethanolamine (pH 6.5), 0.3 mM 2-phosphorglycerate, 0.2 mM beta- NADH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.2 mM ADP, 3.5 unit pyruvate kinase, 5 unit L-lactic dehydrogenas.; 2. Equilibrate to 37C and monitor at A340nm until the value is constant using a spectrophotometer.; 3. Add 20ul of recombinant ENO3 protein 50ug/ml in assay buffer.; 4. Record the decrease in A340 nm for 20 minutes at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ENO3 active protein

Beta-enolase, also known as ENO3, is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. ENO3 play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Recombinant human ENO3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ENO3 active protein

Metabolism

References

Keller A., et al. (1994) J Neurosci Res. 38:493-504.; Zhang E., et al. (1997) Biochemistry. 36:12526-12534.

NCBI and Uniprot Product Information

• NCBI GI #: 16878083
• NCBI GeneID: 2027
• Molecular Weight: 49 kDa (454aa), confirmed by MALDI-TOF
• NCBI Official Full Name: Enolase 3 (beta, muscle)
• NCBI Official Synonym Full Names: enolase 3
• NCBI Official Symbol: ENO3
• NCBI Official Synonym Symbols: MSE; GSD13
• NCBI Protein Information: beta-enolase
• UniProt Protein Name: Beta-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO3
• UniProt Synonym Gene Names: MSE

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]

Uniprot Description

Appears to have a function in striated muscle development and regeneration.