LCN1 recombinant protein

LCN1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120862

• Gene Names: LCN1; TP; TLC; PMFA; VEGP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LCN1; LCN1 Protein; Human; Recombinant (His Tag); Human LCN1/VEGP/Lipocalin-1 Protein (His Tag); Lipocalin 1 Protein; MGC71975 Protein; PMFA Protein; TLC Protein; TP Protein; VEGP Protein; lipocalin 1; LCN1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp176

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human LCN1 (NP_002288.1) extracellular domain (Met 1-Asp176) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LCN1 recombinant protein

Background: Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear Prealbumin, VEGP, Tear lipocalin, and LCN1 is a secreted protein that belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1/VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1/VEGP is identical to lingual von Ebner's gland protein and is also produced in the prostate, nasal mucosa, and tracheal mucosa. Homologous proteins have been found in the rat, pig, and probably dog and horse. Lipocalin-1/VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1/VEGP acts as the principal lipid-binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1/VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1/LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1/LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.

References

Lassagne H. et al., 1993, Exp. Eye Res. 56:605-609. Redl,B. et al., 2000, Biochim Biophys Acta 1482 (1-2):241-8. Wojnar P. et al., 2001, J. Biol. Chem. 276:20206-20212. Wojnar P. et al., 2003, J. Biol. Chem. 278:16209-16215. Breustedt D.A. et al., 2005, J. Biol. Chem. 280:484-493.

NCBI and Uniprot Product Information

• NCBI GI #: 4504963
• NCBI GeneID: 3933
• NCBI Accession #: NP_002288.1
• NCBI GenBank Nucleotide #: NM_002297.3
• UniProt Accession #: P31025; Q5T8A1
• Molecular Weight: 19,250 Da
• NCBI Official Full Name: lipocalin-1 isoform 1
• NCBI Official Synonym Full Names: lipocalin 1
• NCBI Official Symbol: LCN1
• NCBI Official Synonym Symbols: TP; TLC; PMFA; VEGP
• NCBI Protein Information: lipocalin-1; VEG protein; tear prealbumin; Von Ebner gland protein; lipocalin 1 (tear prealbumin); protein migrating faster than albumin
• UniProt Protein Name: Lipocalin-1
• Protein Family: Lipocalin
• UniProt Gene Name: LCN1
• UniProt Synonym Gene Names: VEGP; Tlc; TP; VEG protein
• UniProt Entry Name: LCN1_HUMAN

NCBI Description

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region

Molecular Function: cysteine protease inhibitor activity

Biological Process: long-chain fatty acid transport; retinal homeostasis; sensory perception of taste; response to stimulus; proteolysis; transmembrane transport