Rabbit anti-Human, Mouse ADAMTS18 Polyclonal Antibody | anti-ADAMTS18 antibody

ADAMTS18, CT (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 18, ADAMTS-18, ADAM-TS 18, ADAM-TS18, ADAMTS21) (Azide free) (HRP)

Cat. Num. AAA6461525

• Gene Names: ADAMTS18; KNO2; ADAMTS21
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADAMTS18; Polyclonal Antibody; CT (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 18; ADAMTS-18; ADAM-TS 18; ADAM-TS18; ADAMTS21) (Azide free) (HRP); anti-ADAMTS18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADAMTS18. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ADAMTS18 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1066-1095 in the C-teminal region of human ADAMTS18, UniProt Accession #Q8TE60.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS631215. ADAMTS18 (arrow) was detected usingMBS631215.)

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS631215. This demonstrates that MBS631215 detected the ADAMTS18 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS631215, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS631215 for immunohistochemistry.)

Product Categories/Family for anti-ADAMTS18 antibody

Antibodies; Matrix Metalloproteinases

References

1. Zeng, W., et.al., Biochim. Biophys. Acta 1760(3), 517-524 (2006). 2. Jin, H., et.al., Oncogene 26(53), 7490-7498 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 40806187
• NCBI GeneID: 170692
• NCBI Accession #: NP_955387.1
• NCBI GenBank Nucleotide #: NM_199355.2
• UniProt Accession #: Q8TE60; Q6P4R5; Q6ZWJ9
• Molecular Weight: 135,167 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 18 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 18
• NCBI Official Symbol: ADAMTS18
• NCBI Official Synonym Symbols: KNO2; ADAMTS21
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 18; disintegrin and metalloprotease-like protein; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 18; a disintegrin-like and metalloprotease (reproly
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 18
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS18
• UniProt Synonym Gene Names: ADAMTS21; ADAM-TS 18; ADAM-TS18; ADAMTS-18
• UniProt Entry Name: ATS18_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the protein encoded by gene ADAMTS16, another family member. It is thought to function as a tumor suppressor. Alternatively spliced transcript variants have been identified, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS18: Defects in ADAMTS18 are the cause of Knobloch syndrome type 2 (KNO2). A developmental disorder primarily characterized by typical eye abnormalities, including high myopia, cataracts, dislocated lens, vitreoretinal degeneration, and retinal detachment, with occipital skull defects, which can range from occipital encephalocele to occult cutis aplasia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Secreted; Protease; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: eye development; proteolysis

Disease: Microcornea, Myopic Chorioretinal Atrophy, And Telecanthus