LAMP1 elisa pair set

LAMP1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118360

• Synonyms: LAMP1; LAMP1 Matched ELISA Antibody Pair Set; Human; Human LAMP-1/CD107a/LAMP1 ELISA Pair Set; lysosomal-associated membrane protein 1; CD107a Matched ELISA Antibody Pair Set; LAMPA Matched ELISA Antibody Pair Set; LGP120 Matched ELISA Antibody Pair Set; LAMP1 elisa pair set

• Specificity: Quantitative determination of Human LAMP1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 15.63-1000pg/mL
• Sensitivity: 15.6pg/ml
• Materials Provided: 1. Capture Antibody 0.1 mg/mL of mouse anti-LAMP1 monoclonal antibody, Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL mouse anti-LAMP1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 65 ng of recombinant LAMP1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for LAMP1 elisa pair set

Lysosome-associated membrane glycoprotein 1, also known as CD107 antigen-like family member A, CD107a, and LAMP1, is a single-pass type I membrane protein that belongs to the LAMP family. CD107a is expressed largely in the endosome-lysosome membranes of cells but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD107b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in which amyloid burden was cleared following beta A-peptide immunization. Also, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide, or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.

This LAMP1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LAMP1. It contains Human LAMP1 capture antibody, Human LAMP1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LAMP1 protein.

References

Parkinson-Lawrence EJ, et al. (2005) Immunochemical analysis of CD107a (LAMP-1). Cell Immunol. 236(1-2): 161-6.Barrachina M, et al. (2006) Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 32(5): 505-16.Zhang H, et al. (2010) Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Arch Oral Biol. 55(6): 417-25.