LAMP1 recombinant protein

LAMP1 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123390

• Gene Names: Lamp1; LGP120
• Purity: >92% as determined by SDS-PAGE
• Synonyms: LAMP1; LAMP1 Protein; Rat; Recombinant (hFc Tag); Rat LAMP1/CD107a Protein (Fc Tag); lysosomal-associated membrane protein 1; LAMP1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn371

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the Rat LAMP1 (P14562)(Met1-Asn371) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LAMP1 recombinant protein

Background: Lysosome-associated membrane glycoprotein 1, also known as CD107 antigen-like family member A, CD107a, and LAMP1, is a single-pass type I membrane protein that belongs to the LAMP family. CD107a is expressed largely in the endosome-lysosome membranes of cells but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD107b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in which amyloid burden was cleared following beta A-peptide immunization. Also, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide, or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.

References

Parkinson-Lawrence EJ, et al. (2005) Immunochemical analysis of CD107a (LAMP-1). Cell Immunol. 236(1-2): 161-6.Barrachina M, et al. (2006) Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 32(5): 505-16.Zhang H, et al. (2010) Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Arch Oral Biol. 55(6): 417-25.

NCBI and Uniprot Product Information

• NCBI GI #: 126378
• NCBI GeneID: 25328
• UniProt Accession #: P14562; P97620
• Molecular Weight: 43,969 Da
• NCBI Official Full Name: Lysosome-associated membrane glycoprotein 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 1
• NCBI Official Symbol: Lamp1
• NCBI Official Synonym Symbols: LGP120
• NCBI Protein Information: lysosome-associated membrane glycoprotein 1
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 1
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: Lamp1
• UniProt Synonym Gene Names: Lamp-1; LAMP-1; Lysosome-associated membrane protein 1; LGP-120
• UniProt Entry Name: LAMP1_RAT

NCBI Description

a lysosomal acidic membrane protein [RGD, Feb 2006]

Uniprot Description

LAMP1: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. Belongs to the LAMP family.

Protein type: Membrane protein, integral