Testing Data
CD55/DAF ELISA Pair Set | CD55 elisa pair set
CD55/DAF Matched ELISA Antibody Pair Set, Mouse
Synonyms
CD55/DAF; CD55/DAF Matched ELISA Antibody Pair Set; Mouse; Mouse CD55/DAF ELISA Pair Set; CD55 molecule; decay accelerating factor for complement (Cromer blood group); Daf Matched ELISA Antibody Pair Set; Daf-GPI Matched ELISA Antibody Pair Set; Daf1 Matched ELISA Antibody Pair Set; GPI-DAF Matched ELISA Antibody Pair Set; CD55 elisa pair set
Specificity
Quantitative determination of Mouse CD55/DAF
Assay Type
Solid Phase Sandwich ELISA
Detection Range
5.47-350pg/mL
Sensitivity
5.47pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse CD55/DAF monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse CD55/DAF polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 19 ng of recombinant Mouse CD55/DAF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 350 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse CD55/DAF polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 19 ng of recombinant Mouse CD55/DAF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 350 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CD55 elisa pair set
CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
This CD55/DAF Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD55/DAF. It contains Mouse CD55/DAF capture antibody, Mouse CD55/DAF detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD55/DAF protein.
This CD55/DAF Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD55/DAF. It contains Mouse CD55/DAF capture antibody, Mouse CD55/DAF detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD55/DAF protein.
Product Categories/Family for CD55 elisa pair set
References
Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.
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Product Notes
The CD55 (Catalog #AAA8118504) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD55/DAF, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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