Rabbit anti-Human CD55/DAF Monoclonal Antibody | anti-CD55 antibody

Recombinant Anti-CD55/DAF Antibody (APC), Rabbit Monoclonal

Cat. Num. AAA8100896

• Gene Names: CD55; CR; TC; DAF; CROM
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD55/DAF; Monoclonal Antibody; Recombinant Anti-CD55/DAF Antibody (APC); Rabbit Monoclonal; CD55/DAF Antibody (APC); Rabbit MAb; CD55 molecule; decay accelerating factor for complement (Cromer blood group); Anti-CR Antibody; Anti-CROM Antibody; Anti-DAF Antibody; Anti-TC Antibody; anti-CD55 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 028
• Specificity: Human CD55/DAF
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD55 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD55/DAF protein
• Conjugation: APC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD55/DAF (rh CD55/DAF) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of anti-CD55 reactivity on K562 cells analyzed by flow cytometry.)

Related Product Information for anti-CD55 antibody

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.

Product Categories/Family for anti-CD55 antibody

Complement Activation Pathways

References

Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.

NCBI and Uniprot Product Information

• NCBI GI #: 10835143
• NCBI GeneID: 1604
• NCBI Accession #: NP_000565.1
• NCBI GenBank Nucleotide #: NM_000574.3
• UniProt Accession #: P08174; P09679; P78361; B1AP14; D3DT83; D3DT84
• Molecular Weight: 41,400 Da
• NCBI Official Full Name: complement decay-accelerating factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• NCBI Official Symbol: CD55
• NCBI Official Synonym Symbols: CR; TC; DAF; CROM
• NCBI Protein Information: complement decay-accelerating factor; CD55 antigen
• UniProt Protein Name: Complement decay-accelerating factor
• Protein Family: Complement decay-accelerating factor
• UniProt Gene Name: CD55
• UniProt Synonym Gene Names: CR; DAF
• UniProt Entry Name: DAF_HUMAN

NCBI Description

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft

Molecular Function: viral receptor activity; protein binding; lipid binding

Biological Process: respiratory burst; entry of virus into host cell; elevation of cytosolic calcium ion concentration; negative regulation of complement activation; regulation of complement activation; innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; complement activation, classical pathway

Disease: Blood Group, Cromer System