Mouse CD63 Monoclonal Antibody | anti-CD63 antibody

MOUSE ANTI HUMAN CD63:FITC

Cat. Num. AAA210851

• Gene Names: CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD63; Monoclonal Antibody; MOUSE ANTI HUMAN CD63:FITC; anti-CD63 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MEM-259
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 238

• Applicable Applications for anti-CD63 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Peripheral Human Monocytes stained with Mouse anti Human CD63:APC (MBS215543))

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD63:FITC)

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD63 (MBS212520))

Related Product Information for anti-CD63 antibody

Mouse anti Human CD63 antibody, clone MEM-259 recognizes the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60kDa. CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies (Vischer et al. 1993) and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation (Hamamoto et al. 1994). CD63 expression is also associated with melanoma development (Radford et al. 1997) and expression is rapidly down-regulated by progesterone in endometrial tissues (Okada et al. 1999).

NCBI and Uniprot Product Information

• NCBI GI #: 383872447
• NCBI GeneID: 967
• NCBI Accession #: NP_001244318.1
• NCBI GenBank Nucleotide #: NM_001257389.1
• UniProt Accession #: P08962; Q5TZP3; Q8N6Z9; Q9UCG6; F8VZE2
• Molecular Weight: 17,271 Da
• NCBI Official Full Name: CD63 antigen isoform A
• NCBI Official Synonym Full Names: CD63 molecule
• NCBI Official Symbol: CD63
• NCBI Official Synonym Symbols: MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• NCBI Protein Information: CD63 antigen; CD63 antigen (melanoma 1 antigen); granulophysin; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; ocular melanoma-associated antigen; tetraspanin-30; tspan-30
• UniProt Protein Name: CD63 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: CD63
• UniProt Synonym Gene Names: MLA1; TSPAN30; LAMP-3; Tspan-30
• UniProt Entry Name: CD63_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: cell surface; late endosome membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; melanosome; platelet dense granule membrane; endosome membrane; intrinsic to plasma membrane

Molecular Function: protein binding

Biological Process: platelet activation; protein transport; cell migration; platelet degranulation; pigment granule maturation; cell-matrix adhesion; positive regulation of receptor internalization; blood coagulation