Rabbit anti-Mouse CD55/DAF Monoclonal Antibody | anti-CD55 antibody

Recombinant Anti-CD55/DAF Antibody (PE), Rabbit Monoclonal

Cat. Num. AAA8111615

• Gene Names: Cd55; Daf; Daf1; Daf-GPI; GPI-DAF
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD55/DAF; Monoclonal Antibody; Recombinant Anti-CD55/DAF Antibody (PE); Rabbit Monoclonal; CD55/DAF Antibody (PE); Rabbit MAb; CD55 molecule; decay accelerating factor for complement (Cromer blood group); Anti-Daf Antibody; Anti-Daf-GPI Antibody; Anti-Daf1 Antibody; Anti-GPI-DAF Antibody; anti-CD55 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 076
• Specificity: Mouse CD55/DAF
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD55 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse CD55/DAF protein
• Conjugation: PE
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD55/DAF (rM CD55/DAF) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Mouse CD55 expression on mouse splenocytes. BABL/c splenocytes were stained with PE-conjugated anti-Mouse CD55. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-CD55 antibody

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.

Product Categories/Family for anti-CD55 antibody

Complement Activation Pathways

References

Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.

NCBI and Uniprot Product Information

• NCBI GI #: 114326522
• NCBI GeneID: 13136
• NCBI Accession #: NP_034146.2
• NCBI GenBank Nucleotide #: NM_010016.3
• UniProt Accession #: Q61475; P97732; Q3TU32; Q4FJS4; Q61397; Q76N72; Q921P0; Q9R1C1
• Molecular Weight: 42,618 Da
• NCBI Official Full Name: complement decay-accelerating factor, GPI-anchored preproprotein
• NCBI Official Synonym Full Names: CD55 molecule, decay accelerating factor for complement
• NCBI Official Symbol: Cd55
• NCBI Official Synonym Symbols: Daf; Daf1; Daf-GPI; GPI-DAF
• NCBI Protein Information: complement decay-accelerating factor, GPI-anchored
• UniProt Protein Name: Complement decay-accelerating factor, GPI-anchored
• Protein Family: Complement decay-accelerating factor
• UniProt Gene Name: Cd55
• UniProt Synonym Gene Names: Cd55a; Daf; Daf1; DAF-GPI

NCBI Description

This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]

Uniprot Description

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1 E4|1 56.89 cM

Cellular Component: apical plasma membrane; cell surface; external side of plasma membrane; lipid raft; plasma membrane

Molecular Function: enzyme inhibitor activity; lipid binding; viral receptor activity

Biological Process: regulation of complement activation, classical pathway