CEACAM1 elisa pair set
CEACAM1 Matched ELISA Antibody Pair Set, Human
Synonyms
CEACAM1; CEACAM1 Matched ELISA Antibody Pair Set; Human; Human CEACAM1 ELISA Pair Set; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); BGP Matched ELISA Antibody Pair Set; BGP1 Matched ELISA Antibody Pair Set; BGPI Matched ELISA Antibody Pair Set; CD66a Matched ELISA Antibody Pair Set; CEACAM-1 Matched ELISA Antibody Pair Set; CEACAM1 elisa pair set
Specificity
Quantitative determination of Human CEACAM1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
78.13-5000pg/mL
Sensitivity
78.13pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human CEACAM1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human CEACAM1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.2ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 137 ng of recombinant Human CEACAM1. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 5000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human CEACAM1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.2ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 137 ng of recombinant Human CEACAM1. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 5000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Related Product Information for CEACAM1 elisa pair set
The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
This CEACAM1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CEACAM1. It contains Human CEACAM1 capture antibody, Human CEACAM1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CEACAM1 protein.
This CEACAM1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CEACAM1. It contains Human CEACAM1 capture antibody, Human CEACAM1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CEACAM1 protein.
Product Categories/Family for CEACAM1 elisa pair set
References
Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
