Typical Testing Data/Standard Curve (for reference only)
Tocilizumab ELISA Kit
Tocilizumab (Actemra) (mAb-based) ELISA
Synonyms
Tocilizumab; Tocilizumab (Actemra) (mAb-based) ELISA; Actemra; Tocilizumab elisa kit
Specificity
There is no cross reaction with any other proteins present in native human serum. A screening test was performed with 32 different native human sera. All produced OD450/620nm values less than the mean OD of standard D (6 ng/mL). No cross reaction was observed with sera spiked with the other therapeutic antibodies including Adalimumab, Golimumab, Infliximab, Etanercept, Rituximab, Bevacizumab, Trastuzumab and Omalizumab at concentrations up to 1 mg/mL. All produced OD450/620nm values less than the mean OD of standard D.
Application Notes
1 hour incubation (RT) + 1 hour (RT) + 10 min. (RT) = 2 hours, 10 min. total incubation time.
Samples
Serum and plasma
Intra-assay Precision
<10%.
Inter-assay Precision
<10%.
Preparation and Storage
Store at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Tocilizumab elisa kit
Intended Uses: Enzyme immunoassay for the specific determination of free Tocilizumab in serum and plasma. For research use only -
Principle of the Assay: This ELISA is based on a Tocilizumab-specific monoclonal antibody (catcher Ab, ImmunoGuide clone IG-7C84). Diluted standards and diluted samples are incubated in the microtiter plate coated with IG-7C84 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-Tocilizumab antibody is added and binds to the Tocilizumab. Following incubation, the wells are washed, and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Tocilizumab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Tocilizumab to the solid phase, pre-coated with IG-7C84, is inhibited by recombinant human interleukin-6 receptor-alpha (rh-IL6R?) in a concentration dependent manner. Therefore, the ImmunoGuide Tocilizumab ELISA (mAb-Based) measures the free form of Tocilizumab.
Principle of the Assay: This ELISA is based on a Tocilizumab-specific monoclonal antibody (catcher Ab, ImmunoGuide clone IG-7C84). Diluted standards and diluted samples are incubated in the microtiter plate coated with IG-7C84 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-Tocilizumab antibody is added and binds to the Tocilizumab. Following incubation, the wells are washed, and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Tocilizumab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Tocilizumab to the solid phase, pre-coated with IG-7C84, is inhibited by recombinant human interleukin-6 receptor-alpha (rh-IL6R?) in a concentration dependent manner. Therefore, the ImmunoGuide Tocilizumab ELISA (mAb-Based) measures the free form of Tocilizumab.
Product Categories/Family for Tocilizumab elisa kit
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Product Notes
The Tocilizumab (Catalog #AAA490287) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 1 hour incubation (RT) + 1 hour (RT) + 10 min. (RT) = 2 hours, 10 min. total incubation time. Researchers should empirically determine the suitability of the Tocilizumab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tocilizumab, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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