Western Blot (WB)
(Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).)
Rabbit anti-Human ENTPD3 Polyclonal Antibody | anti-ENTPD3 antibody
ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6) (MaxLight 550)
Gene Names
ENTPD3; HB6; CD39L3; NTPDase-3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
ENTPD3; Polyclonal Antibody; CT (ENTPD3; CD39L3; Ectonucleoside triphosphate diphosphohydrolase 3; CD39 antigen-like 3; Ecto-ATP diphosphohydrolase 3; Ecto-apyrase 3; HB6) (MaxLight 550); HB6; anti-ENTPD3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ENTPD3.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-ENTPD3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 496-527 in the C-terminal region of human ENTPD3.
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646154 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646154 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HL-60 cells using MBS646154 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HL-60 cells using MBS646154 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ENTPD3 antibody
References
1. Fausther, M., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 298 (6), L804-L818 (2010). 2. Gaddie, K.J., et al. Biochemistry 48(40):9437-9447 (2009). 3. Munkonda, M.N., et al. FEBS J. 276(2):479-496 (2009). 4. Munkonda, M.N., et al. Biochem. Pharmacol. 74(10):1524-1534 (2007). 5. Ivanenkov, V.V., et al. Biochemistry 44(25):8998-9012 (2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ectonucleoside triphosphate diphosphohydrolase 3 isoform 1
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 3
NCBI Official Symbol
ENTPD3
NCBI Official Synonym Symbols
HB6; CD39L3; NTPDase-3
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 3
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 3
Protein Family
UniProt Gene Name
ENTPD3
UniProt Synonym Gene Names
CD39L3; NTPDase 3; Ecto-ATPDase 3; Ecto-ATPase 3
UniProt Entry Name
ENTP3_HUMAN
Uniprot Description
ENTPD3: Has a threefold preference for the hydrolysis of ATP over ADP. Belongs to the GDA1/CD39 NTPase family.
Protein type: EC 3.6.1.5; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 3p21.3
Molecular Function: protein binding
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Product Notes
The ENTPD3 entpd3 (Catalog #AAA6295772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENTPD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENTPD3 entpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENTPD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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