Typical Testing Data/Standard Curve (for reference only)
ANGPTL6 elisa kit
ANGPTL6 ELISA Kit
Gene Names
ANGPTL6; AGF; ARP5
Synonyms
ANGPTL6; ANGPTL6 ELISA Kit; Angiopoietin-like protein 6; ANGPTL6 elisa kit
Specificity
The assay recognizes both natural and recombinant human Angiopoietin-2. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested: VEGF-A, GM-CSF, IL-34, IL-29, IFN?, IL-2, IL-10, CD138, MICB, MCP-1.
Sequence Length
470
Samples
Supernatants, Buffered Solutions And Serum Samples
Assay Type
Qualitative Sandwich
Sensitivity
25 pg/ml
Intra-assay Precision
Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of Ang-2 : 2 in human pooled serum, 2 in complete culture media and 2 in standard diluent. 2 standard curves were run on each plate
Inter-assay Precision
Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of Ang-2 : 2 in human pooled serum, 2 in complete culture media and 2 in standard diluent. 2 standard curves were run on each plate.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ANGPTL6 elisa kit
Intended Uses: The Angiopoietin-2 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of Angiopoietin-2 in supernatants, buffered solutions and serum samples. This assay will recognise both natural and recombinant human Angiopoietin-2.
Principle of the Assay: A capture Antibody highly specific for Angiopoietin-2 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Angiopoietin-2 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti-Angiopoietin-2 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Angiopoietin-2 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Angiopoietin-2 in any sample tested.
Principle of the Assay: A capture Antibody highly specific for Angiopoietin-2 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Angiopoietin-2 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti-Angiopoietin-2 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Angiopoietin-2 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Angiopoietin-2 in any sample tested.
Product Categories/Family for ANGPTL6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,694 Da
NCBI Official Full Name
angiopoietin-related protein 6
NCBI Official Synonym Full Names
angiopoietin like 6
NCBI Official Symbol
ANGPTL6
NCBI Official Synonym Symbols
AGF; ARP5
NCBI Protein Information
angiopoietin-related protein 6
UniProt Protein Name
Angiopoietin-related protein 6
Protein Family
UniProt Gene Name
ANGPTL6
UniProt Synonym Gene Names
AGF; ARP5
UniProt Entry Name
ANGL6_HUMAN
Uniprot Description
ANGPTL6: May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: secretory granule
Biological Process: angiogenesis; cell differentiation
Research Articles on ANGPTL6
Similar Products
Product Notes
The ANGPTL6 angptl6 (Catalog #AAA495435) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ANGPTL6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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