Typical Testing Data/Standard Curve (for reference only)
MMP-1 elisa kit
MMP-1 ELISA Kit
Synonyms
MMP-1; MMP-1 ELISA Kit; Matrix metalloproteinase-1; Interstitial Collagenase; Fibroblast Collagenase; MMP-1 elisa kit
Specificity
The assay recognizes both natural and recombinant human MICB. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested: MICA, Angiopoïetin-2, IL-1?, IL-34, IL-10, IL-31, IFN?, GM-CSF, VEGF-165 and IL-2.
Sequence Length
310
Samples
Supernatants, Buffered Solutions Or Serum And Plasma Samples
Assay Type
Qualitative Sandwich
Sensitivity
12.3 pg/ml
Intra-assay Precision
Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of MICB: 2 in human pooled serum, 2 in culture media and 2 in standard diluent. 1 standard curve was run on each plate. Data below show the mean MICB concentration and the coefficient of variation for each sample. The calculated overall coefficient of variation was 4.8%
Inter-assay Precision
Assay to assay reproducibility within one laboratory was evaluated in three independent experiments by two technicians. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of MICB: 2 in human pooled serum, 2 in culture media and 2 in standard diluent. 1 standard curve was run on each plate. Data below show the mean MICB concentration and the coefficient of variation for each sample.
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MMP-1 elisa kit
Intended Uses: The MICB ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of MICB in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human MICB.
Principle of the Assay: A capture Antibody highly specific for MICB has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of MICB samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-MICB secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of MICB present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of MICB in any sample tested.
Principle of the Assay: A capture Antibody highly specific for MICB has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of MICB samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-MICB secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of MICB present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of MICB in any sample tested.
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NCBI and Uniprot Product Information
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Product Notes
The MMP-1 apicbibun_04585 (Catalog #AAA495391) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MMP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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