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Typical Testing Data/Standard Curve (for reference only)

General 8-epi-PGF2alpha ELISA Kit

8-epi-PGF2alpha ELISA Kit

Reactivity
General
Synonyms
8-epi-PGF2alpha; 8-epi-PGF2alpha ELISA Kit; 15-F2t-IsoP; 8-iso-PGF2a; 8-isoprostane; 8-iso-PG; 8-iso Prostaglandin; 8-epi-PGF2alpha elisa kit
Ordering
For Research Use Only!
Reactivity
General
Specificity
This kit recognizes 8-epi-PGF2alpha in samples. No Significant cross-reactivity or interference between 8-epiPGF2alpha and analogues was observed.
Detection Range
15.63—1000 pg/mL
Sensitivity
9.38 pg/mL
Preparation and Storage
An unopened kit can be stored at 2-8 degree C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received..
Shelf life: ~12 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 8-epi-PGF2alpha elisa kit
Background/Introduction: F2?isoprostanes are a family of prostaglandin F2?like compounds that are formed by free?radical?catalyzed peroxidation of arachidonic acid. Several F2?isoprostanes, but in particular 8?epi PGF2alpha, are widely used as oxidative stress biomarkers. High levels of F2-IPs can disrupt the physicochemical integrity of cell membranes but more importantly can exert significant pharmacological activity. 8-epi-PGF2alpha is potent vasoconstrictor acting on the renal, pulmonary, and retinal vasculatures, modulates platelet aggregation and is involved in the activation of intracellular signaling. The 8-epi-PGF2alpha ELISA Kit is based on the Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with 8-epi-PGF2alpha. During the reaction, 8-epi-PGF2alphain the samples or standard competes with 8-epi-PGF2alpha coated on the plate for binding to the anti-8-epi-PGF2alphaantibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microplate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of 8-epi-PGF2alpha. The concentration of 8-epi-PGF2alpha in the samples can be calculated by comparing the OD of the samples to the standard curve.

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Product Notes

The General 8-epi-PGF2alpha (Catalog #AAA849494) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849494 ELISA Kit recognizes General 8-epi-PGF2alpha. It is sometimes possible for the material contained within the vial of "8-epi-PGF2alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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