Typical Testing Data/Standard Curve (for reference only)
HFABP/FABP3 elisa kit
HFABP/FABP3 ELISA
Synonyms
HFABP/FABP3; HFABP/FABP3 ELISA; Heart-type fatty acid binding protein (hFABP); mammary-derived growth inhibitor; HFABP/FABP3 elisa kit
Application Notes
90 min. incubation (37 degree C) + 60 min. (37 degree C) + 30 min. (37 degree C) = 3 hours total incubation time.
Samples
Serum, cell culture supernatants or tissue homogenate
Assay Type
Sandwich
Detection Range
15.6 pg/ml - 1000 pg/ml
Sensitivity
1 pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, or at -20 degree C for one year. Store HRP conjugated antibody at 4 degree C for one year.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HFABP/FABP3 elisa kit
Intended Uses: Sandwich enzyme-linked immune-sorbent assay for the determination of human HFABP in serum, cell culture supernatants or tissue homogenate.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HFABP monoclonal antibody is pre-coated onto 96-well plates. The standards and test samples are added to the wells and any HFABP present is bound by the immobilized antibody. After washing away any unbound substances, the HRP conjugated anti-HFABP monoclonal antibody is added to wells as detection antibody. Following a wash to remove any unbound antibody- enzyme reagent, the TMB substrates are added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding stop solution. The density of yellow is proportional to the HFABP amount of sample captured in plate. The O.D. absorbance is read at 450nm using a microplate reader, and then the concentration of HFABP can be calculated.
Background: Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The serum HFABP level is an effective biomarker for detecting AKI in nonsurgical intensive care subjects. HFABP expression is significantly higher in in-stent restenosis, ISR tissue than in non- ISR tissue from diabetic and nondiabetic minipigs. Overexpression of HFABP induces multiple pathway-related promotion of inflammation, growth and migration in vascular SMCs, suggesting a potential role in coronary artery ISR. hFABP is a rapid marker of perioperative myocardial damage and peaks earlier than CKMB or TnI. The kinetics of marker proteins in serial samples immediately after reperfusion is more suitable for the detection of perioperative myocardial infarction than a fixed cut-off level.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HFABP monoclonal antibody is pre-coated onto 96-well plates. The standards and test samples are added to the wells and any HFABP present is bound by the immobilized antibody. After washing away any unbound substances, the HRP conjugated anti-HFABP monoclonal antibody is added to wells as detection antibody. Following a wash to remove any unbound antibody- enzyme reagent, the TMB substrates are added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding stop solution. The density of yellow is proportional to the HFABP amount of sample captured in plate. The O.D. absorbance is read at 450nm using a microplate reader, and then the concentration of HFABP can be calculated.
Background: Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The serum HFABP level is an effective biomarker for detecting AKI in nonsurgical intensive care subjects. HFABP expression is significantly higher in in-stent restenosis, ISR tissue than in non- ISR tissue from diabetic and nondiabetic minipigs. Overexpression of HFABP induces multiple pathway-related promotion of inflammation, growth and migration in vascular SMCs, suggesting a potential role in coronary artery ISR. hFABP is a rapid marker of perioperative myocardial damage and peaks earlier than CKMB or TnI. The kinetics of marker proteins in serial samples immediately after reperfusion is more suitable for the detection of perioperative myocardial infarction than a fixed cut-off level.
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Product Notes
The HFABP/FABP3 (Catalog #AAA490250) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 90 min. incubation (37 degree C) + 60 min. (37 degree C) + 30 min. (37 degree C) = 3 hours total incubation time. Researchers should empirically determine the suitability of the HFABP/FABP3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFABP/FABP3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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