Typical Testing Data/Standard Curve (for reference only)
Cardiac Troponin I (cTnI) ELISA Kit | cTnI elisa kit
Cardiac Troponin I (cTnI) ELISA
Synonyms
Cardiac Troponin I (cTnI); Cardiac Troponin I (cTnI) ELISA; cTnI elisa kit
Sequence Length
189
Application Notes
90 min. incubation (37 degree C) + 60 min. (37 degree C) + 30 min. (37 degree C) = 3 hours total incubation time.
Samples
Human cTnI in serum, cell culture supernatants or tissue homogenate
Assay Type
Sandwich
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
10 pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, or at -20 degree C for one year. Store HRP conjugated antibody at 4 degree C for one year.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for cTnI elisa kit
Intended Uses: Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of human cTnI in serum, cell culture supernatants or tissue homogenate.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-cTnI monoclonal antibody is precoated onto 96-well plates. The standards and test samples are added to the wells and any cTnI present is bound by the immobilized antibody. After washing away any unbound substances, the HRP conjugated anti-cTnI monoclonal antibody is added to wells as the detection antibody. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates are added to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding stop solution. The density of yellow is proportional to the cTnI amount of sample captured in plate. The O.D. absorbance is read at 450nm using a microplate reader, and then the concentration of cTnI can be calculated.
Background: Troponin I is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle, the others are troponin T and troponin C. Human troponin I is presented in cardiac muscle tissue by a single isoform with molecular weight 23876 Da and it consists of 209 amino acid residues. It binds actin and inhibits actomyosin ATPase activity in the absence of calcium. In 2000, Labarrere et al. found that persistent elevation of cardiac troponin I levels after heart transplantation were associated with high risk for developing coronary artery disease and graft failure after cardiac transplantation. The cardiac troponin I may be a useful tool in identifying subjects with heart failure who are at increased risk for progressive ventricular dysfunction and death.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-cTnI monoclonal antibody is precoated onto 96-well plates. The standards and test samples are added to the wells and any cTnI present is bound by the immobilized antibody. After washing away any unbound substances, the HRP conjugated anti-cTnI monoclonal antibody is added to wells as the detection antibody. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates are added to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding stop solution. The density of yellow is proportional to the cTnI amount of sample captured in plate. The O.D. absorbance is read at 450nm using a microplate reader, and then the concentration of cTnI can be calculated.
Background: Troponin I is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle, the others are troponin T and troponin C. Human troponin I is presented in cardiac muscle tissue by a single isoform with molecular weight 23876 Da and it consists of 209 amino acid residues. It binds actin and inhibits actomyosin ATPase activity in the absence of calcium. In 2000, Labarrere et al. found that persistent elevation of cardiac troponin I levels after heart transplantation were associated with high risk for developing coronary artery disease and graft failure after cardiac transplantation. The cardiac troponin I may be a useful tool in identifying subjects with heart failure who are at increased risk for progressive ventricular dysfunction and death.
Product Categories/Family for cTnI elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cardiac troponin I
NCBI Official Symbol
LOC100462680
NCBI Protein Information
cardiac troponin I
Research Articles on cTnI
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Product Notes
The cTnI (Catalog #AAA490249) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 90 min. incubation (37 degree C) + 60 min. (37 degree C) + 30 min. (37 degree C) = 3 hours total incubation time. Researchers should empirically determine the suitability of the cTnI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cardiac Troponin I (cTnI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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