Typical Testing Data/Standard Curve (for reference only)
IL-1R1 elisa kit
IL-1R1 ELISA
Gene Names
Il1r1; IL-iR; CD121a; CD121b; IL-1R1; Il1r-1
Synonyms
IL-1R1; IL-1R1 ELISA; Interleukin-1 receptor; type I; IL-1R1 elisa kit
Sequence Length
573
Application Notes
90 min. incubation (37 degree C) + 60 min. (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 3 hours, 30 min. total incubation time.
Samples
Human serum, plasma, cell culture supernatants or tissue homogenate
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
<2 pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-1R1 elisa kit
Intended Uses: Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of IL-1R1 in human serum, plasma, cell culture supernatants or tissue homogenate.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1R1 polyclonal antibody is precoated onto 96-well plates and the biotin conjugated anti-IL-1R1 polyclonal antibody is used as the detection antibody. The standards, test samples, and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the IL- 1R1 amount of sample captured in plate. The O.D. absorbance is read at 450nm using a microplate reader, and then the concentration of IL-1R1 can be calculated.
Background: Interleukin 1 receptor, type I (IL1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL1R1 also denotes its human gene. Helicobacter pylori (Hp) is an environmental inducer of gastritis and gastric cancer (GC). The immune response to Hp and the associated changes in somatic gene expression are key determinants governing the transition from gastritis to GC. Disruption of the HNF4alpha/IL-1R1/IL-1beta/NF-kappaB circuit during Hp infection maybe an effective means of preventing the associated GC. Signaling through the IL-1-receptor type 1 (IL-1R1), IL-1 is required for initiation and maintenance of diverse activities of the immune system. A second receptor, IL-1R2, blocks IL-1 signal transduction. The functions of the IL-1R2 as a "decoy-receptor" sequestrating paracrine IL-1 extracellularly and intracellularly by engaging IL-1IRAcP, thus depriving IL1-R1 molecules of their extracellular and intracellular ligands. Expression of IL1-R2 by HRS cells seems to contribute to local and systemic modulation of immune function in HL.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1R1 polyclonal antibody is precoated onto 96-well plates and the biotin conjugated anti-IL-1R1 polyclonal antibody is used as the detection antibody. The standards, test samples, and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the IL- 1R1 amount of sample captured in plate. The O.D. absorbance is read at 450nm using a microplate reader, and then the concentration of IL-1R1 can be calculated.
Background: Interleukin 1 receptor, type I (IL1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL1R1 also denotes its human gene. Helicobacter pylori (Hp) is an environmental inducer of gastritis and gastric cancer (GC). The immune response to Hp and the associated changes in somatic gene expression are key determinants governing the transition from gastritis to GC. Disruption of the HNF4alpha/IL-1R1/IL-1beta/NF-kappaB circuit during Hp infection maybe an effective means of preventing the associated GC. Signaling through the IL-1-receptor type 1 (IL-1R1), IL-1 is required for initiation and maintenance of diverse activities of the immune system. A second receptor, IL-1R2, blocks IL-1 signal transduction. The functions of the IL-1R2 as a "decoy-receptor" sequestrating paracrine IL-1 extracellularly and intracellularly by engaging IL-1IRAcP, thus depriving IL1-R1 molecules of their extracellular and intracellular ligands. Expression of IL1-R2 by HRS cells seems to contribute to local and systemic modulation of immune function in HL.
Product Categories/Family for IL-1R1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,628 Da
NCBI Official Full Name
interleukin-1 receptor type 1 isoform 2
NCBI Official Synonym Full Names
interleukin 1 receptor, type I
NCBI Official Symbol
Il1r1
NCBI Official Synonym Symbols
IL-iR; CD121a; CD121b; IL-1R1; Il1r-1
NCBI Protein Information
interleukin-1 receptor type 1
UniProt Protein Name
Interleukin-1 receptor type 1
UniProt Gene Name
Il1r1
UniProt Synonym Gene Names
Il-1r1; Il1ra; IL-1R-1; IL-1RT-1; IL-1RT1; IL-1R-alpha; mIL-1R1; mIL-1RI; sIL-1R1; sIL-1RI
Uniprot Description
Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex ().
Research Articles on IL-1R1
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Product Notes
The IL-1R1 il1r1 (Catalog #AAA490248) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 90 min. incubation (37 degree C) + 60 min. (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 3 hours, 30 min. total incubation time. Researchers should empirically determine the suitability of the IL-1R1 il1r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1R1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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