Typical Testing Data/Standard Curve (for reference only)
Creatine Kinase MB (CK-MB) ELISA Kit | CK-MB elisa kit
Creatine Kinase MB (CK-MB) ELISA
Synonyms
Creatine Kinase MB (CK-MB); Creatine Kinase MB (CK-MB) ELISA; CK-MB elisa kit
Application Notes
90 min. incubation (37 degree C) + 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 3 hours, 30 min. total incubation time.
Samples
Human serum, plasma, tissue homogenates or cell culture supernatants
Assay Type
Sandwich
Detection Range
3.12 ng/ml-200 ng/ml
Preparation and Storage
Store at 2-8 degree C for 2 months, or at -20 degree C for 4 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CK-MB elisa kit
Intended Uses: Enzyme-linked immune-sorbent assay (ELISA) for the determination of CK-MB in human serum, plasma, tissue homogenates or cell culture supernatants.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CK-MB monoclonal antibody is pre-coated onto 96-well plates. The biotin conjugated anti-CK-MB monoclonal antibody is used as detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-MB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK-MB can be calculated.
Background: Creatine kinase isoenzyme-MB (CK-MB) is a biomarker for detecting myocardial injury. It is a simple, widely available, and useful biomarker for predicting adverse outcomes in pulmonary embolism. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The CK-MB-tototal- CK ratio is an easily available indicator and could be utilized as a primary screening tool for cancer. Higher ratio of CK-MB-to-total-CK was specifically associated with certain malignancies, like colorectal cancer, lung cancer and hepatocellular carcinoma, as well as some cancer-associated status factors such as advanced stage and liver metastasis.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CK-MB monoclonal antibody is pre-coated onto 96-well plates. The biotin conjugated anti-CK-MB monoclonal antibody is used as detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-MB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK-MB can be calculated.
Background: Creatine kinase isoenzyme-MB (CK-MB) is a biomarker for detecting myocardial injury. It is a simple, widely available, and useful biomarker for predicting adverse outcomes in pulmonary embolism. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The CK-MB-tototal- CK ratio is an easily available indicator and could be utilized as a primary screening tool for cancer. Higher ratio of CK-MB-to-total-CK was specifically associated with certain malignancies, like colorectal cancer, lung cancer and hepatocellular carcinoma, as well as some cancer-associated status factors such as advanced stage and liver metastasis.
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Product Notes
The CK-MB (Catalog #AAA490245) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 90 min. incubation (37 degree C) + 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 3 hours, 30 min. total incubation time. Researchers should empirically determine the suitability of the CK-MB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Creatine Kinase MB (CK-MB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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