Typical Testing Data/Standard Curve (for reference only)
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E Coli O157:H7 ELISA Kit
E Coli O157:H7 ELISA
Synonyms
E Coli O157:H7; E Coli O157:H7 ELISA; Escherichia coli O157:H7; E Coli O157:H7 elisa kit
Application Notes
90 min. incubation (37 degree C) + 1 hour (37 degree C) + 30 min. (37 degree C) = 3 hours total incubation time.
Samples
Food or water
Assay Type
Sandwich
Detection Range
6.25×103 - 4×10 5 cfu/ml
Sensitivity
< 1 ×10^3 cfu/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months. Store HRP conjugated antibody at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for E Coli O157:H7 elisa kit
Intended Uses: Enzyme-linked immune-sorbent assay for the determination of E.coli O157:H7 in food or water. For research use only,
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-E.coli O157:H7 monoclonal antibody is pre-coated onto 96-well plates. The HRP conjugated anti-E.coli O157:H7 polyclonal antibody is used as detection antibodies. The standards, test samples and HRP conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the E.coli O157:H7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of E.coli O157:H7 can be calculated.
Background/Introduction: Escherichia coli O157:H7 is an enterohemorrhagic serotype of the bacterium Escherichia coli and a cause of illness, typically through consumption of contaminated food. As an emerging pathogen, Escherichia coli O157:H7 causes severe enteritis and the extraintestinal complication of hemolytic-uremic syndrome. Rokhsartalab-Azar S et al evaluate the conjugate of E. coli O157: H7 lipopolysaccharide (LPS) with diphtheria toxoid (DT) as a candidate vaccine in mice model. Their results showed that the suggested vaccine composed of E. coli O157:H7 LPS and DT had a significant potential to protect against E. coli infections. Infection with Escherichia coli O157:H7 may develop into hemorrhagic colitis, or hemolytic uremic syndrome (HUS), which usually causes kidney failure or even death. The adhesion and toxins are the important virulent factors. Cai K et al constructed a novel vaccine candidate rSOBGs based on the bacterial ghost (BG). They found the novel vaccine candidate rSOBGs induced both anti-toxin and anti-adhesion immune protection, suggesting the possibility to prevent the infectious diseases caused by Escherichia coli O157:H7.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-E.coli O157:H7 monoclonal antibody is pre-coated onto 96-well plates. The HRP conjugated anti-E.coli O157:H7 polyclonal antibody is used as detection antibodies. The standards, test samples and HRP conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the E.coli O157:H7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of E.coli O157:H7 can be calculated.
Background/Introduction: Escherichia coli O157:H7 is an enterohemorrhagic serotype of the bacterium Escherichia coli and a cause of illness, typically through consumption of contaminated food. As an emerging pathogen, Escherichia coli O157:H7 causes severe enteritis and the extraintestinal complication of hemolytic-uremic syndrome. Rokhsartalab-Azar S et al evaluate the conjugate of E. coli O157: H7 lipopolysaccharide (LPS) with diphtheria toxoid (DT) as a candidate vaccine in mice model. Their results showed that the suggested vaccine composed of E. coli O157:H7 LPS and DT had a significant potential to protect against E. coli infections. Infection with Escherichia coli O157:H7 may develop into hemorrhagic colitis, or hemolytic uremic syndrome (HUS), which usually causes kidney failure or even death. The adhesion and toxins are the important virulent factors. Cai K et al constructed a novel vaccine candidate rSOBGs based on the bacterial ghost (BG). They found the novel vaccine candidate rSOBGs induced both anti-toxin and anti-adhesion immune protection, suggesting the possibility to prevent the infectious diseases caused by Escherichia coli O157:H7.
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Product Notes
The E Coli O157:H7 (Catalog #AAA490246) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 90 min. incubation (37 degree C) + 1 hour (37 degree C) + 30 min. (37 degree C) = 3 hours total incubation time. Researchers should empirically determine the suitability of the E Coli O157:H7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E Coli O157:H7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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