Typical Testing Data/Standard Curve (for reference only)
Human Elastase 1, Pancreatic ELISA Kit | ELA1 elisa kit
Human ELA1 (Elastase 1, Pancreatic) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ELA1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ELA1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ELA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ELA1.You can calculate the concentration of ELA1in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]
Uniprot Description
CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.36; Secreted, signal peptide; Secreted; Protease
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: extracellular space
Molecular Function: metal ion binding; serine-type endopeptidase activity
Biological Process: exocrine pancreas development; inflammatory response; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; proteolysis; regulation of cell differentiation; regulation of cell proliferation; tissue remodeling; Wnt receptor signaling pathway
Research Articles on ELA1
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Product Notes
The Human ELA1 cela1 (Catalog #AAA2533416) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533416 ELISA Kit recognizes Human ELA1. It is sometimes possible for the material contained within the vial of "Elastase 1, Pancreatic, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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