Typical Testing Data/Standard Curve (for reference only)
Monkey Osteocalcin ELISA Kit | OC elisa kit
Monkey Osteocalcin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: OCN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OCN antibody and an OCN-HRP conjugate. The assay sample and buffer are incubated together with OCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OCN concentration since OCN from samples and OCN-HRP conjugate compete for the anti-OCN antibody binding site. Since the number of sites is limited, as more sites are occupied by OCN from the sample, fewer sites are left to bind OCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OCN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Uniprot Description
osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.
Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon
Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity
Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to ethanol; response to vitamin D; regulation of osteoclast differentiation; response to vitamin K; response to mechanical stimulus; response to zinc ion; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization
Research Articles on OC
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Product Notes
The Monkey OC bglap (Catalog #AAA738559) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738559 ELISA Kit recognizes Monkey OC. It is sometimes possible for the material contained within the vial of "Osteocalcin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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