SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
EGF-L6 recombinant protein
Recombinant EGF-L6
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
EGF-L6; Recombinant EGF-L6; Epidermal growth factor-like protein 6; Egfl6; Maeg; EGF-L6 recombinant protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
287-550aa
Sequence
ADLTMKKKVK LKMVTPRPAS TRVPKVNLPY SSEEGVSRGR NYDGEQKKKE EGKRERLEEE KGEKTLRNEV EQERTLRGDV FSPKVNEAED LDLVYVQRKE LNSKLKHKDL NISVDCSFDL GVCDWKQDRE DDFDWHPADR DNDVGYYMAV PALAGHKKNI GRLKLLLPNL TPQSNFCLLF DYRLAGDKVG KLRVFVKNSN NALAWEETKN EDGRWRTGKI QLYQGIDTTK SVIFEAERGK GKTGEIAVDG VLLVSGLCPD DFLSVEGHHH HHH
Sequence Length
550
Applicable Applications for EGF-L6 recombinant protein
SDS-PAGE
Species
Mouse
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ala
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for EGF-L6 recombinant protein
EGF-L6, also known as epidermal growth factor-like protein 6, is a member of the EGF repeat superfamily of proteins. This protein is expressed in fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta. This protein is expressed by epidermal stem cells in the hair follicle bulge and play a role in hair follicle morphogenesis through binding integrin alpha-8/beta-1. It is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity. Recombinant mouse EGF-L6 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for EGF-L6 recombinant protein
References
Wang X., et al, (2012) PLoS One. 7:e52707. Bai S., et al, (2016) Cancer Res. 76:6396-6409.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31.1kDa (273aa); 28-40KDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
epidermal growth factor-like protein 6
UniProt Protein Name
Epidermal growth factor-like protein 6
UniProt Gene Name
Egfl6
UniProt Synonym Gene Names
Maeg; EGF-like protein 6
UniProt Entry Name
EGFL6_MOUSE
Uniprot Description
EGFL6: May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; membrane; extracellular region; basement membrane
Molecular Function: calcium ion binding
Biological Process: extracellular matrix organization and biogenesis; multicellular organismal development; cell adhesion; cell differentiation
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Product Notes
The EGF-L6 egfl6 (Catalog #AAA201125) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 287-550aa. AAA Biotech's EGF-L6 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the EGF-L6 egfl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADLTMKKKVK LKMVTPRPAS TRVPKVNLPY SSEEGVSRGR NYDGEQKKKE EGKRERLEEE KGEKTLRNEV EQERTLRGDV FSPKVNEAED LDLVYVQRKE LNSKLKHKDL NISVDCSFDL GVCDWKQDRE DDFDWHPADR DNDVGYYMAV PALAGHKKNI GRLKLLLPNL TPQSNFCLLF DYRLAGDKVG KLRVFVKNSN NALAWEETKN EDGRWRTGKI QLYQGIDTTK SVIFEAERGK GKTGEIAVDG VLLVSGLCPD DFLSVEGHHH HHH. It is sometimes possible for the material contained within the vial of "EGF-L6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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