IL2RB recombinant protein

Recombinant IL2RB

Cat. Num. AAA201087

• Gene Names: IL2RB; CD122; IMD63; IL15RB; P70-75
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: IL2RB; Recombinant IL2RB; Interleukin 2 receptor subunit beta; CD122; IL15RB; P70-75; IL2RB recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 27-240aa
• Sequence: AVNGTSQFTC FYNSRANISC VWSQDGALQD TSCQVHAWPD RRRWNQTCEL LPVSQASWAC NLILGAPDSQ KLTTVDIVTL RVLCREGVRW RVMAIQDFKP FENLRLMAPI SLQVVHVETH RCNISWEISQ ASHYFERHLE FEARTLSPGH TWEEAPLLTL KQKQEWICLE TLTPDTQYEF QVRVKPLQGE FTTWSPWSQP LAFRTKPAAL GKDTHHHHHH
• Sequence Length: 551

• Applicable Applications for IL2RB recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala27
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for IL2RB recombinant protein

IL2RB, also known as interleukin 2 receptor subunit beta, is a member of the cytokine receptor superfamily. This protein, which is involved in T cell-mediated immune responses, is present in three forms with respect to ability to bind interleukin 2. It denoted IL2RA, IL2RB, and the common cytokine receptor gamma chain, which is shared by the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. The low affinity form is a monomer of the alpha subunit (also called CD25) and is not involved in signal transduction. The intermediate affinity form consists of a gamma/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. Recombinant human IL2RB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL2RB recombinant protein

Immunology; Hormones & Cytokines

References

Jounaidi Y., et al, (2017) Cancer Res. 77:5938-5951. Purvis SF., et al, (1992) Cell. Immunol. 144:32-42.

NCBI and Uniprot Product Information

• NCBI GI #: 4504665
• NCBI GeneID: 3560
• NCBI Accession #: NP_000869.1
• NCBI GenBank Nucleotide #: NP_000869.1
• UniProt Accession #: P14784
• Molecular Weight: 25.5kDa (220aa); 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: interleukin-2 receptor subunit beta
• NCBI Official Synonym Full Names: interleukin 2 receptor subunit beta
• NCBI Official Symbol: IL2RB
• NCBI Official Synonym Symbols: CD122; IMD63; IL15RB; P70-75
• NCBI Protein Information: interleukin-2 receptor subunit beta
• UniProt Protein Name: Interleukin-2 receptor subunit beta
• Protein Family: Interleukin-2 receptor
• UniProt Gene Name: IL2RB
• UniProt Synonym Gene Names: IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; p75
• UniProt Entry Name: IL2RB_HUMAN

NCBI Description

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. The use of alternative promoters results in multiple transcript variants encoding the same protein. The protein is primarily expressed in the hematopoietic system. The use by some variants of an alternate promoter in an upstream long terminal repeat (LTR) results in placenta-specific expression. [provided by RefSeq, Sep 2016]

Uniprot Description

IL2RB: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation. Interacts with HTLV-1 accessory protein p12I. Belongs to the type I cytokine receptor family. Type 4 subfamily.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: interleukin-2 receptor activity; interleukin-2 binding

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; protein complex assembly; signal transduction; negative regulation of apoptosis