IL-15 active protein

Recombinant IL-15

Cat. Num. AAA201109

• Gene Names: IL15; IL-15
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: IL-15; Recombinant IL-15; Interleukin-15; ILO0701; IL-15 active protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffer Saline (pH7.4) Containing 10% Glycerol.
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 49-162 aa
• Sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECEELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH
• Sequence Length: 162

• Applicable Applications for IL-15 active protein: Bioactivity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Biological Activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for IL-15 active protein

Interleukin (IL) -15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma (c) -expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant human IL-15 was expressed in E Coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

Product Categories/Family for IL-15 active protein

Hormones & Cytokines; Signal Transduction; Immunology

References

J G Giri., et al. (1994) EMBO. 13(12): 2822-2830. Lorenzen I., et al. (2006). J Biol Chem. 281(10):6642-7.

NCBI and Uniprot Product Information

• NCBI GI #: 10835153
• NCBI GeneID: 3600
• NCBI Accession #: NP_000576.1
• NCBI GenBank Nucleotide #: NP_000576.1
• UniProt Accession #: P40933
• Molecular Weight: 13.9kDa (123aa) confirmed by MALDI-TOF
• NCBI Official Full Name: interleukin-15 isoform 1 preproprotein
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: IL15
• NCBI Official Synonym Symbols: IL-15
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• UniProt Gene Name: IL15
• UniProt Synonym Gene Names: IL-15
• UniProt Entry Name: IL15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]