EDA2R recombinant protein

EDA2R Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120997

• Gene Names: EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• Purity: >95% as determined by SDS-PAGE
• Synonyms: EDA2R; EDA2R Protein; Human; Recombinant (hFc Tag); Human XEDAR/EDA2R Protein (Fc Tag); EDA-A2R Protein; EDAA2R Protein; TNFRSF27 Protein; XEDAR Protein; ectodysplasin A2 receptor; EDA2R recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu136

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human EDA2R (NP_068555.1)(Met1-Glu136) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EDA2R recombinant protein

Background: Tumor necrosis factor receptor superfamily member 27, also known as X-linked ectodysplasin-A2 receptor, EDA-A2 receptor, EDA2R, XEDAR and TNFRSF27, is a single-pass type III membrane protein. TNFRSF27/EDA2R contains threeTNFR-Cys repeats. It is a new member of the tumor necrosis factor receptor family that has been shown to be highly expressed in ectodermal derivatives during embryonic development and binds to ectodysplasin-A2 (EDA-A2). TNFRSF27/EDA2R is a receptor for EDA isoform A2, but not for EDA isoform A1. TNFRSF27/EDA2R mediates the activation of the NF-kappa-B and JNK pathways. The activation seems to be mediated by binding to TRAF3 and TRAF6. Ectodysplasin, a member of the tumor necrosis factor family, is encoded by the anhidrotic ectodermal dysplasia EDA gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that differ only by an insertion of two amino acids. This insertion functions to determine receptor binding specificity, such that EDA-A1 binds only the receptor EDAR, whereas EDA-A2 binds only the related, but distinct, X-linked ectodysplasin-A2 receptor (XEDAR).

References

Yan M., et al., 2000,Science. 290: 523-527. Sinha S.K., et al., 2002, J. Biol. Chem. 277: 44953-44961. Prodi, D.A. et al., 2008, J Invest Dermatol 128 (9):2268-70.

NCBI and Uniprot Product Information

• NCBI GI #: 11140823
• NCBI GeneID: 60401
• NCBI Accession #: NP_068555.1
• NCBI GenBank Nucleotide #: NM_021783.3
• UniProt Accession #: Q9HAV5; Q5VYX9; Q5VYY0; Q6UWM2; Q8IZA6
• Molecular Weight: 32,959 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 27 isoform 1
• NCBI Official Synonym Full Names: ectodysplasin A2 receptor
• NCBI Official Symbol: EDA2R
• NCBI Official Synonym Symbols: XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; X-linked ectodysplasin-A2 receptor; tumor necrosis factor receptor superfamily member XEDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 27
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: EDA2R
• UniProt Synonym Gene Names: TNFRSF27; XEDAR; EDA-A2 receptor
• UniProt Entry Name: TNR27_HUMAN

NCBI Description

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

EDA2R: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: integral to plasma membrane; integral to membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; receptor activity

Biological Process: epidermis development; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; positive regulation of JNK cascade; activation of NF-kappaB transcription factor