Rabbit anti-Human EDA2R Polyclonal Antibody | anti-EDA2R antibody

EDA2R, CT (EDA2R, TNFRSF27, XEDAR, Tumor necrosis factor receptor superfamily member 27, X-linked ectodysplasin-A2 receptor) (Biotin)

Cat. Num. AAA6294315

• Gene Names: EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EDA2R; Polyclonal Antibody; CT (EDA2R; TNFRSF27; XEDAR; Tumor necrosis factor receptor superfamily member 27; X-linked ectodysplasin-A2 receptor) (Biotin); X-linked ectodysplasin-A2 receptor; anti-EDA2R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EDA2R antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EDA2R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-297 amino acids from the C-terminal region of human EDA2R.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EDA2R Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the EDA2R Antibody detected the EDA2R protein (arrow).)

Related Product Information for anti-EDA2R antibody

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Multiple alternatively spliced transcript variants have been found for this gene, but some variants lack sufficient support. [provided by RefSeq].

Product Categories/Family for anti-EDA2R antibody

Antibodies; Disease Markers

References

Brockschmidt, F.F., et al. Br. J. Dermatol. 162(4):899-903(2010). Tanikawa, C., et al. Oncogene 28(34):3081-3092(2009). Fujimoto, A., et al. J. Hum. Genet. 54(8):461-465(2009). Hillmer, A.M., et al. Hum. Genet. 126(2):255-264(2009). Richards, J.B., et al. Nat. Genet. 40(11):1282-1284(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 11140823
• NCBI GeneID: 60401
• NCBI Accession #: NP_068555.1
• NCBI GenBank Nucleotide #: NM_021783.3
• UniProt Accession #: Q9HAV5; Q5VYX9; Q5VYY0; Q6UWM2; Q8IZA6
• Molecular Weight: 32,959 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 27 isoform 1
• NCBI Official Synonym Full Names: ectodysplasin A2 receptor
• NCBI Official Symbol: EDA2R
• NCBI Official Synonym Symbols: XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; X-linked ectodysplasin-A2 receptor; tumor necrosis factor receptor superfamily member XEDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 27
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: EDA2R
• UniProt Synonym Gene Names: TNFRSF27; XEDAR; EDA-A2 receptor
• UniProt Entry Name: TNR27_HUMAN

NCBI Description

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

EDA2R: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: integral to plasma membrane; integral to membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; receptor activity

Biological Process: epidermis development; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; positive regulation of JNK cascade; activation of NF-kappaB transcription factor