S100A9 Protein Recombinant Protein | S100A9 recombinant protein

Recombinant Rat S100A9 Protein (His Tag)

Cat. Num. AAA2569442

• Gene Names: S100a9; Mrp14
• Purity: >85% as determined by reducing SDS-PAGE
• Synonyms: S100A9 Protein; Recombinant Rat S100A9 Protein (His Tag); Protein S100-A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9; CAGB; CFAG; MRP14; S100A9 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4
• Sequence: Ala2-Lys113

• Species: Rat
• Tag: N-His
• Protein Construction: A DNA sequence encoding the Rat S100A9 (P50116-1)(Ala2-Lys113) was expressed with a polyhistidine tag at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

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Related Product Information for S100A9 recombinant protein

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Has transnitrosylase activity; in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3. The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif.

NCBI and Uniprot Product Information

• NCBI GI #: 16758364
• NCBI GeneID: 94195
• NCBI Accession #: NP_446039.1
• NCBI GenBank Nucleotide #: NM_053587.1
• UniProt Accession #: P50116; Q761U7
• Molecular Weight: 13,145 Da
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9
• NCBI Official Symbol: S100a9
• NCBI Official Synonym Symbols: Mrp14
• NCBI Protein Information: protein S100-A9; p14; MRP-14; calgranulin-B; myeloid-related protein 14; intracellular calcium-binding protein (MRP14); migration inhibitory factor-related protein 14; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9 (calgr
• UniProt Protein Name: Protein S100-A9
• Protein Family: Protein
• UniProt Gene Name: S100a9
• UniProt Synonym Gene Names: Mrp14; MRP-14; p14
• UniProt Entry Name: S10A9_RAT

NCBI Description

calcium binding protein that may be associated with acute inflammatory processes [RGD, Feb 2006]

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Calcium-binding

Cellular Component: extracellular space; cytoskeleton; cytoplasm; plasma membrane; nucleus

Molecular Function: antioxidant activity; arachidonic acid binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; response to ethanol; response to zinc ion; leukocyte chemotaxis; actin cytoskeleton reorganization; autophagy; innate immune response; response to lipopolysaccharide; positive regulation of peptide secretion; regulation of integrin biosynthetic process; leukocyte migration during inflammatory response; positive regulation of inflammatory response