Ectodysplasin A (EDA) Recombinant Protein | EDA recombinant protein

Recombinant Ectodysplasin A (EDA)

Cat. Num. AAA2011107

• Gene Names: Eda; Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ectodysplasin A (EDA); Recombinant Ectodysplasin A (EDA); EDA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG S-PDEKAYSEEE SRRVRRNKRS KSGEGADGPV KNKKKGKKAG PPGPNGPPGP PGPPGPQGPP GIPGIPGIPG TTVMGPPGPP GPPGPQGPPG LQGPSGAADK TGTRENQPAV VHLQGQGSAI QVKNDLSGGV LNDWSRITMN PKVFKLHPRS GELEVLVDGT YFIYSQVEVY YINFTDFASY EVVVDEKPFL QCTRSIETGK TNYNTCYTAG VCLLKARQKI AVKMVHADIS INMSKHTT
• Sequence Length: 389

• Applicable Applications for EDA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Pro141~Thr378 (Accession # O54693) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 295789018
• NCBI GeneID: 13607
• NCBI Accession #: NP_001171408.1
• NCBI GenBank Nucleotide #: NM_001177937.1
• UniProt Accession #: O54693; O35705; Q9QWJ8; Q9QZ01; Q9QZ02; B7ZMT7
• Molecular Weight: 26.5kDa
• NCBI Official Full Name: ectodysplasin-A isoform 2
• NCBI Official Synonym Full Names: ectodysplasin-A
• NCBI Official Symbol: Eda
• NCBI Official Synonym Symbols: Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2
• NCBI Protein Information: ectodysplasin-A; EDA protein homolog
• UniProt Protein Name: Ectodysplasin-A
• Protein Family: Ectodysplasin
• UniProt Gene Name: Eda
• UniProt Synonym Gene Names: Ed1; Ta
• UniProt Entry Name: EDA_MOUSE

Uniprot Description

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Cellular Component: endoplasmic reticulum membrane; collagen; membrane; apical part of cell; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: tumor necrosis factor receptor binding

Biological Process: skin development; positive regulation of I-kappaB kinase/NF-kappaB cascade; hair follicle development; pigmentation; cell-matrix adhesion; multicellular organismal development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; cell differentiation; odontogenesis of dentine-containing teeth