Rabbit Activated Leukocyte Cell Adhesion Molecule ELISA Kit | ALCAM elisa kit

Rabbit Activated Leukocyte Cell Adhesion Molecule ELISA Kit

Cat. Num. AAA720353

• Gene Names: ALCAM; MEMD; CD166
• Reactivity: Rabbit
• Synonyms: Activated Leukocyte Cell Adhesion Molecule; Rabbit Activated Leukocyte Cell Adhesion Molecule ELISA Kit; ALCAM elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ALCAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ALCAM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALCAM elisa kit

Principle of the Assay: ALCAM ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALCAM. Standards or samples are then added to the microtiter plate wells and ALCAM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALCAM present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ALCAM are added to each well to "sandwich" the ALCAM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALCAM and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALCAM concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ALCAM elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 886258
• NCBI GeneID: 214
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: alcam
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: ALCAM
• UniProt Synonym Gene Names: MEMD
• UniProt Entry Name: CD166_HUMAN

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion