Human interleukin 29 (interferon, lambda 1) ELISA Kit | IL29 elisa kit

Human Interleukin 29, IL-29 ELISA Kit

Cat. Num. AAA701011

• Gene Names: IFNL1; IL29; IL-29
• Reactivity: Human
• Synonyms: interleukin 29 (interferon; lambda 1); Human Interleukin 29; IL-29 ELISA Kit; Human Interleukin 29 (IL-29) ELISA Kit; IFNL1; IL-29; interferon; lambda 1; interleukin 29; IL29 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IL-29. No significant cross-reactivity or interference between human IL-29 and analogues was observed.
• Sequence Length: 200

• Samples: Serum, plasma, urine, cerebrospinal fluid (CSF), cell culture supernates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of human IL-29 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL29 elisa kit

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-29 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-29 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-29 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-29 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 26024325
• NCBI GeneID: 282618
• NCBI Accession #: NP_742152.1
• NCBI GenBank Nucleotide #: NM_172140.1
• UniProt Accession #: Q8IU54; Q17R34; A0AV25
• Molecular Weight: 21,898 Da
• NCBI Official Full Name: interferon lambda-1
• NCBI Official Synonym Full Names: interferon, lambda 1
• NCBI Official Symbol: IFNL1
• NCBI Official Synonym Symbols: IL29; IL-29
• NCBI Protein Information: interferon lambda-1; IFN-lambda-1; interleukin-29; cytokine Zcyto21; interferon-lambda-1; interleukin 29 (interferon, lambda 1)
• UniProt Protein Name: Interferon lambda-1
• UniProt Gene Name: IFNL1
• UniProt Synonym Gene Names: IL29; ZCYTO21; IFN-lambda-1; IL-29
• UniProt Entry Name: IFNL1_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.