ECE-2 recombinant protein

Recombinant Human ECE-2 Protein (Fc tag)

Cat. Num. AAA2546414

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: ECE-2; Recombinant Human ECE-2 Protein (Fc tag); ECE2; hCG_2022032; KIAA0604; MGC17664; MGC2408; MGC78487; ECE-2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 883

• Application Notes: The recombinant human Fc/ECE2 is a disulfide-linked homodimeric protein. The reduced monomer consists of 921 amino acids and predicts a molecular mass of 105 kDa. As a result of glycosylation, the apparent molecular mass of rh ECE2/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ECE-2 recombinant protein

Background: Endothelin-converting enzyme 2, also known as ECE-2, is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE-2 is found primarily in neural tissues, with high levels of expression in midbrain, cerebellum, hypothalamus, frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.

Description: A DNA sequence encoding the ectodomain of human endothelin converting enzyme 2 isoform A (NP_055508.3) (Gly 199-Trp 883) was fused with the Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 153945761
• NCBI GeneID: 9718
• NCBI Accession #: NP_055508.3
• NCBI GenBank Nucleotide #: NM_014693.3
• UniProt Accession #: O60344; Q6NTG7; Q6UW36; Q8NFD7; Q96NX3; Q96NX4; Q9BRZ8; A5PLK8
• Molecular Weight: 91,211 Da
• NCBI Official Full Name: endothelin-converting enzyme 2 isoform A
• NCBI Official Synonym Full Names: endothelin converting enzyme 2
• NCBI Official Symbol: ECE2
• NCBI Protein Information: endothelin-converting enzyme 2
• UniProt Protein Name: Endothelin-converting enzyme 2
• UniProt Gene Name: ECE2
• UniProt Synonym Gene Names: KIAA0604; ECE-2

NCBI Description

This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ECE2: Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta- amyloid peptide accumulation in brain. May also have methyltransferase activity. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.71; Membrane protein, integral; Methyltransferase, protein arginine, predicted; Protease; Vesicle

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: cytoplasmic vesicle membrane

Molecular Function: metalloendopeptidase activity; protein binding

Biological Process: brain development; cardioblast differentiation; cell-cell signaling; heart development; peptide hormone processing