Western Blot (WB)
(All lanes: Glucokinase antibody at 8ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52kDaObserved band size: 52kDa)
Rabbit anti-Human Glucokinase Polyclonal Antibody | anti-GCK antibody
Rabbit anti-human Glucokinase polyclonal Antibody(GCK)
IHC:1:20-1:200
Western Blot (WB)
(All lanes: Glucokinase antibody at 8ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52kDaObserved band size: 52kDa)
Western Blot (WB)
(All lanes: Glucokinase antibody at 8ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52kDaObserved band size: 52kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine using MBS1493646 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine using MBS1493646 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreas using MBS1493646 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreas using MBS1493646 at dilution 1:100)
NCBI and Uniprot Product Information
NCBI Description
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]
Uniprot Description
GK: a glycolytic enzyme that catalyzes glucose metabolism in the liver and pancreatic beta cells. Acts as a ""glucose sensor"" in beta cells. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. Glucokinase traps glucose inside the cell by catalyzing its phosphorylation to produce glucose-6-phosphate. Has a lower affinity for glucose than the three other isozymes of hexokinase, allowing other organs such as the brain and muscles to have first call on glucose when its supply is limited. Unlike other hexokinases, glucokinase is not inhibited by glucose-6-phosphate. Glucokinase is found in the outer membrane compartment of mitochondria. May bind VDAC, suppressing mitochondrial function. Glucokinase transcription is induced by insulin, perhaps via the activation of Stat 5B. Mutant glucokinase causes a rare form of diabetes and may also play a role in type 2 diabetes. Three splice variant isoforms of human glucokinase have been described.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - galactose; Mitochondrial; Kinase, other; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 7p15.3-p15.1
Cellular Component: actin filament; basal cortex; cytosol; mitochondrion; nucleoplasm; secretory granule
Molecular Function: ADP binding; ATP binding; fructokinase activity; glucokinase activity; glucose binding; magnesium ion binding; mannokinase activity; protein binding; protein phosphatase binding
Biological Process: carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; cellular response to glucose starvation; cellular response to insulin stimulus; detection of glucose; elevation of cytosolic calcium ion concentration; endocrine pancreas development; fructose 2,6-bisphosphate metabolic process; glucose 6-phosphate metabolic process; glucose homeostasis; glucose metabolic process; glucose transport; glycogen biosynthetic process; glycolysis; hexose transport; lipid homeostasis; NADP metabolic process; negative regulation of epinephrine secretion; negative regulation of gluconeogenesis; positive regulation of glycogen biosynthetic process; positive regulation of glycolysis; positive regulation of insulin secretion; regulation of glycolysis; regulation of insulin secretion; regulation of potassium ion transport; second-messenger-mediated signaling; transmembrane transport
Disease: Diabetes Mellitus, Noninsulin-dependent; Diabetes Mellitus, Permanent Neonatal; Hyperinsulinemic Hypoglycemia, Familial, 3; Maturity-onset Diabetes Of The Young, Type 2
Research Articles on GCK
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Product Notes
The GCK gck (Catalog #AAA1493646) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Glucokinase polyclonal Antibody(GCK) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glucokinase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:200-1000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the GCK gck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucokinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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