Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat flightless-interacting protein 1 (LRRFIP1) Standard Curve.)
Human Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1) ELISA Kit | LRRFIP1 elisa kit
Human Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1) ELISA Kit
Gene Names
LRRFIP1; GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
Reactivity
Human
Synonyms
Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1); Human Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1) ELISA Kit; LRRFIP1; FLAP-1; FLIIAP1; GCF-2; GCF2; HUFI-1; MGC10947; MGC119738; MGC119739; TRIP; GC-binding factor 2; NEDD8-conjugating enzyme; transcription factor 9-like; LRRFIP1 elisa kit
Reactivity
Human
Specificity
Human Leucine-rich repeat flightless-interacting protein 1 (LRRFIP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRRFIP1. No significant cross-reactivity or interference between Human LRRFIP1 and analogues was observed.
Sequence Length
640
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat flightless-interacting protein 1 (LRRFIP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat flightless-interacting protein 1 (LRRFIP1) Standard Curve.)
Related Product Information for LRRFIP1 elisa kit
Description: This Human Leucine-rich repeat flightless-interacting protein 1 (LRRFIP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LRRFIP1 in samples. An antibody specific for LRRFIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRRFIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRFIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRFIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The GCF2 cDNA contains an internal region with 98% nucleotide identity with the first 309 bp of the GCF cDNA, but the remainder of the 2 sequences share no significant homology. The predicted 752-amino acid GCF2 protein has a pI of 4.4. The authors confirmed the calculated molecular mass of 83 kD by mass spectroscopy. However, GCF2 migrates as a 160-kD protein by SDS-PAGE. the discrepancy between the calculated and observed masses is due either to the acidic nature of the protein or to an unusual ability of GCF2 to form very stable dimers. GCF2 binds to EGFR promoter fragments in vitro. Cotransfection assays showed that GCF2 represses transcription from 3 different promoters, including that of EGFR.
Background: The GCF2 cDNA contains an internal region with 98% nucleotide identity with the first 309 bp of the GCF cDNA, but the remainder of the 2 sequences share no significant homology. The predicted 752-amino acid GCF2 protein has a pI of 4.4. The authors confirmed the calculated molecular mass of 83 kD by mass spectroscopy. However, GCF2 migrates as a 160-kD protein by SDS-PAGE. the discrepancy between the calculated and observed masses is due either to the acidic nature of the protein or to an unusual ability of GCF2 to form very stable dimers. GCF2 binds to EGFR promoter fragments in vitro. Cotransfection assays showed that GCF2 represses transcription from 3 different promoters, including that of EGFR.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
leucine-rich repeat flightless-interacting protein 1 isoform 1
NCBI Official Synonym Full Names
LRR binding FLII interacting protein 1
NCBI Official Symbol
LRRFIP1
NCBI Official Synonym Symbols
GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
NCBI Protein Information
leucine-rich repeat flightless-interacting protein 1
UniProt Protein Name
Leucine-rich repeat flightless-interacting protein 1
Protein Family
UniProt Gene Name
LRRFIP1
UniProt Synonym Gene Names
GCF2; TRIP; LRR FLII-interacting protein 1
UniProt Entry Name
LRRF1_HUMAN
Uniprot Description
LRRFIP1: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. Belongs to the LRRFIP family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: cytoskeleton; cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; double-stranded RNA binding
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; negative regulation of transcription, DNA-dependent
Research Articles on LRRFIP1
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Product Notes
The Human LRRFIP1 lrrfip1 (Catalog #AAA9715441) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715441 ELISA Kit recognizes Human LRRFIP1. It is sometimes possible for the material contained within the vial of "Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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