Thioredoxin 2 Active Protein | TXN2 active protein

Thioredoxin 2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121188

• Gene Names: TXN2; MTRX; TRX2; MT-TRX
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Thioredoxin 2; Thioredoxin 2 Protein; Human; Recombinant (His Tag); Human Thioredoxin-2/TXN2 Protein (His Tag); MT-TRX Protein; MTRX Protein; RP5-1119A7. 13-005 Protein; TRX2 Protein; thioredoxin 2; TXN2 active protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH8.3. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr60-Gly166

• Species: Human
• Activity: Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650nM. The specific activity is 5-8 A650/min/mg.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human TXN2 (Q99757)(Thr 60-Gly 166) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TXN2 active protein

Background: Thioredoxin-2, also known as TXN2, MTRX and TRX2, is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which, as trypanothione:peroxiredoxin oxidoreductases, constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2/TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2/TXN2 is a small redox protein important in cellular antioxidant defenses, as well as in the regulation of apoptosis. Thioredoxin-2/TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2/TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2/TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2/TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.

References

Chen Y., et al., 2002, J. Biol. Chem. 277: 33242-8. Smeets A., et al., 2005, Protein Sci. 14: 2610-21. Wen,S. et al., 2009, Am J Med Genet A 149A (2): 155-60. Choudhary C., et al., 2009, Science 325: 834-40.

NCBI and Uniprot Product Information

• NCBI GI #: 21361403
• NCBI GeneID: 25828
• NCBI Accession #: NP_036605.2
• NCBI GenBank Nucleotide #: NM_012473.3
• UniProt Accession #: Q99757; Q5JZA0; Q6FH60; Q9UH29
• Molecular Weight: 18,383 Da
• NCBI Official Full Name: thioredoxin, mitochondrial
• NCBI Official Synonym Full Names: thioredoxin 2
• NCBI Official Symbol: TXN2
• NCBI Official Synonym Symbols: MTRX; TRX2; MT-TRX
• NCBI Protein Information: thioredoxin, mitochondrial; mitochondrial thioredoxin; thioredoxin-2
• UniProt Protein Name: Thioredoxin, mitochondrial
• UniProt Gene Name: TXN2
• UniProt Synonym Gene Names: TRX2; MTRX; Mt-Trx
• UniProt Entry Name: THIOM_HUMAN

NCBI Description

This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TXN2: Has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Could be involved in the resistance to anti-tumor agents. Possesses a dithiol-reducing activity. Belongs to the thioredoxin family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cell soma; mitochondrion; mitochondrial matrix; dendrite; nucleolus

Molecular Function: peptide-methionine-(S)-S-oxide reductase activity; protein disulfide oxidoreductase activity; protein complex binding; peptide-methionine (R)-S-oxide reductase activity

Biological Process: response to drug; response to reactive oxygen species; cellular response to nutrient levels; cell redox homeostasis; response to glucose stimulus; response to hormone stimulus; response to hypoxia; response to axon injury; response to organic cyclic substance; response to nutrient; glycerol ether metabolic process