Testing Data #1
(Staining of staurosporine-induced THP-1 cells using the Magic RedTM Cathepsin L kit)
CATHEPSIN L Assay Kit | CatL assay kit
MAGIC RED CATHEPSIN L KIT
Applications
Immunofluorescence
Synonyms
CATHEPSIN L; MAGIC RED CATHEPSIN L KIT; CatL assay kit
Form/Format
Kit
Sequence Length
306
Applicable Applications for CatL assay kit
Immunofluorescence (IF)
Reagents In The Kits
Magic Red MR-(FR)2 Reagent - lyophilized
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
Preparation and Storage
Store the unopened kit (and each unopened component) at 4 degree C until the expiration date.
Protect the MR-(FR)2 reagent from light at all times.
Once reconstituted, the 260X MR-(FR)2 stock should be stored at -20 degree C protected from light.
Shelf Life: The 260X MR-(FR)2 reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
Protect the MR-(FR)2 reagent from light at all times.
Once reconstituted, the 260X MR-(FR)2 stock should be stored at -20 degree C protected from light.
Shelf Life: The 260X MR-(FR)2 reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
Testing Data #1
(Staining of staurosporine-induced THP-1 cells using the Magic RedTM Cathepsin L kit)
Testing Data #1
(Staining of staurosporine-induced THP-1 cells using the Magic RedTM Cathepsin L kit)
Related Product Information for CatL assay kit
Description: The Magic Red Cathepsin L kit uses a quick and easy method to analyze intracellular Cathepsin L protease activity in whole living cells.
Test Principle: Cathepsin Magic Red kits measure Cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic Red reagent contains a Cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Test Principle: Cathepsin Magic Red kits measure Cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic Red reagent contains a Cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CATHEPSIN L catl (Catalog #AAA239203) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CATHEPSIN L can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). Researchers should empirically determine the suitability of the CATHEPSIN L catl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CATHEPSIN L, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
