MCP-1 (MCAF, CCL2) active protein
Rat MCP-1 (MCAF, CCL2)
Gene Names
Ccl2; MCP-1; Scya2; Sigje
Reactivity
Rat
Purity
> 97% (SDS-PAGE; HPLC)
Synonyms
MCP-1 (MCAF; CCL2); Rat MCP-1 (MCAF; Recombinant Rat MCP-1; CCL2) active protein
Host
E Coli
Reactivity
Rat
Purity/Purification
> 97% (SDS-PAGE; HPLC)
Form/Format
Lyophilized
Sequence
QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTK LKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA P LNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN
Sequence Length
148
Endotoxin Level
< 0.1 ng/ug of MCP-1
Biological Activity
Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
Related Product Information for MCP-1 (MCAF, CCL2) active protein
Rat CCL2 is a member of the beta (CC) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best known as a chemotactic agent for mononuclear cells. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. Additionally, it is believed to reduce IL 12 production by dendritic cells and promote a Th2 phenotype in CD4+ T cells. The role of CCL2 in recruiting monocytes to sites of inflammation is implicated in the pathology of multiple sclerosis, atherosclerosis and allergic asthma.
Product Categories/Family for MCP-1 (MCAF, CCL2) active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.0 kDa
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
Ccl2
NCBI Official Synonym Symbols
MCP-1; Scya2; Sigje
NCBI Protein Information
C-C motif chemokine 2; small inducible gene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
Ccl2
UniProt Synonym Gene Names
Je; Mcp1; Scya2; MCP-1
UniProt Entry Name
CCL2_RAT
NCBI Description
a monocyte chemoattractant protein [RGD, Feb 2006]
Uniprot Description
Function: Chemotactic factor that attracts monocytes, but not neutrophils.
Subunit structure: Monomer or homodimer; in equilibrium
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
