MCP-1 (MCAF, CCL2) active protein
Rat MCP-1 (MCAF, CCL2)
Gene Names
Ccl2; MCP-1; Scya2; Sigje
Reactivity
Rat
Purity
> 97% (SDS-PAGE; HPLC)
Synonyms
MCP-1 (MCAF; CCL2); Rat MCP-1 (MCAF; Recombinant Rat MCP-1; CCL2) active protein
Host
E Coli
Reactivity
Rat
Purity/Purification
> 97% (SDS-PAGE; HPLC)
Form/Format
Lyophilized
Sequence
QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTK LKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA P LNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN
Sequence Length
148
Endotoxin Level
< 0.1 ng/ug of MCP-1
Biological Activity
Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
Related Product Information for MCP-1 (MCAF, CCL2) active protein
Rat CCL2 is a member of the beta (CC) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best known as a chemotactic agent for mononuclear cells. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. Additionally, it is believed to reduce IL 12 production by dendritic cells and promote a Th2 phenotype in CD4+ T cells. The role of CCL2 in recruiting monocytes to sites of inflammation is implicated in the pathology of multiple sclerosis, atherosclerosis and allergic asthma.
Product Categories/Family for MCP-1 (MCAF, CCL2) active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.0 kDa
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
Ccl2
NCBI Official Synonym Symbols
MCP-1; Scya2; Sigje
NCBI Protein Information
C-C motif chemokine 2; small inducible gene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
Ccl2
UniProt Synonym Gene Names
Je; Mcp1; Scya2; MCP-1
UniProt Entry Name
CCL2_RAT
NCBI Description
a monocyte chemoattractant protein [RGD, Feb 2006]
Uniprot Description
Function: Chemotactic factor that attracts monocytes, but not neutrophils.
Subunit structure: Monomer or homodimer; in equilibrium
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
Research Articles on MCP-1 (MCAF, CCL2)
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Product Notes
The MCP-1 (MCAF, CCL2) ccl2 (Catalog #AAA691703) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Rat MCP-1 (MCAF, CCL2) reacts with Rat and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTK LKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA P LNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN. It is sometimes possible for the material contained within the vial of "MCP-1 (MCAF, CCL2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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