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Typical Testing Data/Standard Curve (for reference only)

Rat MCP-1/CCL2 ELISA Kit | CCL2 elisa kit

Rat MCP-1/CCL2 PicoKine ELISA Kit

Gene Names
Ccl2; MCP-1; Scya2; Sigje
Reactivity
Rat
Synonyms
MCP-1/CCL2; Rat MCP-1/CCL2 PicoKine ELISA Kit; C-C motif chemokine 2; chemokine (C-C motif) ligand 2; CCL2; CCL2_HUMAN; Chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig-je); Small inducible cytokine subfamily A (Cys-Cys); member 2; Small-inducible cytokine A2; SMC-CF; CCL2 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
Natural and recombinant Rat Ccl2
Sequence Length
148
Samples
Cell culture supernates and serum
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCL2 elisa kit
Principle of the Assay: The Picokine™ Rat Ccl2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ccl2 with a 96-well strip plate that is pre-coated with antibody specific for Ccl2. The detection antibody is a biotinylated antibody specific for Ccl2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ccl2 with immunogen: Expression system for standard: NSO; Immunogen sequence: Q24-N148. The kit is analytically validated with ready to use reagents. To measure Rat Ccl2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Ccl2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl2 in the sample.

Background/Introduction: Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,460 Da
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
Ccl2
NCBI Official Synonym Symbols
MCP-1; Scya2; Sigje
NCBI Protein Information
C-C motif chemokine 2; small inducible gene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE
UniProt Protein Name
C-C motif chemokine 2
Protein Family
UniProt Gene Name
Ccl2
UniProt Synonym Gene Names
Je; Mcp1; Scya2; MCP-1
UniProt Entry Name
CCL2_RAT

NCBI Description

a monocyte chemoattractant protein [RGD, Feb 2006]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Cellular Component: extracellular space; rough endoplasmic reticulum; cell soma; perinuclear region of cytoplasm; endocytic vesicle; cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; G-protein-coupled receptor binding; chemokine activity; CCR2 chemokine receptor binding

Biological Process: positive regulation of cell adhesion; maternal process involved in pregnancy; positive regulation of leukocyte migration; response to glucocorticoid stimulus; response to lipopolysaccharide; response to antibiotic; monocyte chemotaxis; regulation of cell shape; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; response to drug; neutrophil chemotaxis; organ regeneration; response to amino acid stimulus; positive regulation of tumor necrosis factor production; response to ethanol; cellular response to insulin stimulus; response to bacterium; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemokinesis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; positive regulation of cell-cell adhesion; protein kinase B signaling cascade; response to wounding; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; inflammatory response; lymphocyte chemotaxis; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Research Articles on CCL2

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Product Notes

The Rat CCL2 ccl2 (Catalog #AAA176509) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176509 ELISA Kit recognizes Rat CCL2. It is sometimes possible for the material contained within the vial of "MCP-1/CCL2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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