Non-Metastatic Cells 2 Active Protein | NME2 active protein

Recombinant Human Non-Metastatic Cells 2

Cat. Num. AAA141636

• Gene Names: NME1-NME2; NMELV; NM23-LV
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Non-Metastatic Cells 2; Recombinant Human Non-Metastatic Cells 2; NME2; NME2 Human; Non-Metastatic Cells 2 Human Recombinant; Nucleoside diphosphate kinase B; NDPK-B; NDPKB; NM23-H2; NM23B; NME2 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The NME2 solution (1mg/ml) contains 10% glycerol, 20mM Tris-HCl buffer (pH8.0) and 1mM DTT.
• Sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

• Biological Activity: Specific activity is >1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH7.5 at 25 degree C in a couple system with PK/LDH.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for NME2 active protein

Non-Metastatic Cells 2 or NME2, is a protein, that acts as a nucleoside diphosphate kinase. This protein is of a heterodimeric structure and has 152 aa A & B polypeptide chains that builds the whole protein. Erythrocyte NDP kinase beta subunit has an identical structure to NME2. NDP kinases linked to nucleoside triphosphates synthesis and it appears as though NME2 takes part in regulating signal transduction.
NME2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.2kDa.

Product Categories/Family for NME2 active protein

RECOMBINANT & NATURAL PROTEINS

NCBI and Uniprot Product Information

• NCBI GI #: 66392203
• NCBI GeneID: 654364
• NCBI Accession #: NP_001018146.1
• NCBI GenBank Nucleotide #: NM_001018136.2
• UniProt Accession #: P22392; Q1WM23; Q6LCT6; A8MWA3
• Molecular Weight: 17,298 Da
• NCBI Official Full Name: NME1-NME2 protein
• NCBI Official Synonym Full Names: NME1-NME2 readthrough
• NCBI Official Symbol: NME1-NME2
• NCBI Official Synonym Symbols: NMELV; NM23-LV
• NCBI Protein Information: NME1-NME2 protein; NME1-NME2 readthrough transcript
• UniProt Protein Name: Nucleoside diphosphate kinase B
• UniProt Gene Name: NME2
• UniProt Synonym Gene Names: NM23B; NDK B; NDP kinase B
• UniProt Entry Name: NDKB_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring NME1 and NME2 genes. The significance of this read-through transcription and the function of the resulting protein product have not yet been determined. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Nucleotide Metabolism - pyrimidine; EC 2.7.13.3; Nucleotide Metabolism - purine; Nuclear receptor co-regulator; EC 2.7.4.6; Kinase, nucleoside diphosphate; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; membrane; mitochondrion; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; cytosol; nucleus

Molecular Function: gamma-tubulin binding; protein binding; protein histidine kinase activity; metal ion binding; nucleoside diphosphate kinase activity; intermediate filament binding; protein kinase binding; transcription factor activity; ATP binding; single-stranded DNA binding

Biological Process: integrin-mediated signaling pathway; GTP biosynthetic process; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of myeloid leukocyte differentiation; UTP biosynthetic process; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; positive regulation of epithelial cell proliferation; regulation of epidermis development; response to amine stimulus; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; negative regulation of apoptosis