sRANK active protein

Human sRANK (Receptor)

Cat. Num. AAA696086

• Gene Names: TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• Reactivity: Species Reactivity: Human; Cross Reactivity: Mouse
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Synonyms: sRANK; Human sRANK (Receptor); Recombinant Human sRANK Receptor; sRANK active protein

• Host: E Coli
• Reactivity: Species Reactivity: Human; Cross Reactivity: Mouse
• Purity/Purification: Above 98% as determined by SDS-PAGE Analysis.
• Sequence: MQIAPPCTS EKHYEHLGR CCNKCEPGK YMSSKCTTT SDSVCLPCG PDEYLDSWN EEDKCLLHK VCDTGKALV AVVAGNSTT PRRCACTAG YHWSQDCEC CRRNTECAP GLGAQHPLQ LNKDTVCKP CLAGYFSDA FSSTDKCRP WTNCTFLGK RVEHHGTEK SDAVCSSSL PARK

• Biological Activity: Determined by its ability to inhibit sRANKL induced NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect in the presence of 15ng/ml of recombinant sRANKL, is 30-50 ng/ml.
• Cell Type: T Cells, Dendritic Cells
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for sRANK active protein

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cell-mediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.3 kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (175 amino acid residues).

Product Categories/Family for sRANK active protein

Angiogenesis; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 19924309
• NCBI GeneID: 8792
• UniProt Accession #: Q9Y6Q6; Q59EP9; I4EC36; I4EC38; I4EC39; I7JE63; N0GVH0
• Molecular Weight: 64,552 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 11A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: TNFRSF11A
• NCBI Official Synonym Symbols: FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A; Paget disease of bone 2; receptor activator of NF-KB; osteoclast differentiation factor receptor; receptor activator of nuclear factor-kappa B; loss of heterozygosity, 18, chromosomal region 1
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• UniProt Gene Name: TNFRSF11A
• UniProt Synonym Gene Names: RANK; ODFR
• UniProt Entry Name: TNR11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]

Uniprot Description

TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q22.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; tumor necrosis factor receptor activity; cytokine binding; metal ion binding; receptor activity

Biological Process: circadian thermoregulation; positive regulation of JNK activity; adaptive immune response; ossification; response to lipopolysaccharide; osteoclast differentiation; signal transduction; lymph node development; activation of NF-kappaB transcription factor; monocyte chemotaxis; response to radiation; tumor necrosis factor-mediated signaling pathway; cell-cell signaling; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of transcription factor activity

Disease: Osteopetrosis, Autosomal Recessive 7; Familial Expansile Osteolysis; Paget Disease Of Bone