Rabbit anti-Human FAAH2 Polyclonal Antibody | anti-FAAH2 antibody

FAAH2, CT (FAAH2, AMDD, Fatty-acid amide hydrolase 2, Amidase domain-containing protein, Anandamide amidohydrolase 2, Oleamide hydrolase 2) (FITC)

Cat. Num. AAA6297562

• Gene Names: FAAH2; AMDD
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FAAH2; Polyclonal Antibody; CT (FAAH2; AMDD; Fatty-acid amide hydrolase 2; Amidase domain-containing protein; Anandamide amidohydrolase 2; Oleamide hydrolase 2) (FITC); Oleamide hydrolase 2; anti-FAAH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FAAH2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FAAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 416-446 amino acids from the C-terminal region of human FAAH2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FAAH2 Antibody (C-term) in HepG2 cell line lysates (35ug/lane).FAAH2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(FAAH2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FAAH2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FAAH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FAAH2 antibody

FAAH2 encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. The encoded enzyme is able to catalyze the hydrolysis of a broad range of bioactive lipids, including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl amino acids. This enzyme has a preference for monounsaturated acyl chains as a substrate.

Product Categories/Family for anti-FAAH2 antibody

Antibodies; Enzymes

References

Kaczocha, M., et al. J. Biol. Chem. 285(4):2796-2806(2010). Karbarz, M.J., et al. Anesth. Analg. 108(1):316-329(2009). Wei, B.Q., et al. J. Biol. Chem. 281(48):36569-36578(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 195972892
• NCBI GeneID: 158584
• NCBI Accession #: NP_777572.2
• NCBI GenBank Nucleotide #: NM_174912.3
• UniProt Accession #: Q6GMR7; Q86VT2; Q96N98
• Molecular Weight: 58,304 Da
• NCBI Official Full Name: fatty-acid amide hydrolase 2
• NCBI Official Synonym Full Names: fatty acid amide hydrolase 2
• NCBI Official Symbol: FAAH2
• NCBI Official Synonym Symbols: AMDD
• NCBI Protein Information: fatty-acid amide hydrolase 2; amidase domain containing; amidase domain-containing protein; anandamide amidohydrolase 2; oleamide hydrolase 2
• UniProt Protein Name: Fatty-acid amide hydrolase 2
• Protein Family: Fatty-acid amide hydrolase
• UniProt Gene Name: FAAH2
• UniProt Synonym Gene Names: AMDD
• UniProt Entry Name: FAAH2_HUMAN

NCBI Description

This gene encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. The encoded enzyme is able to catalyze the hydrolysis of a broad range of bioactive lipids, including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl amino acids. This enzyme has a preference for monounsaturated acyl chains as a substrate.[provided by RefSeq, Sep 2009]

Uniprot Description

FAAH2: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes monounsaturated substrate anandamide preferentially as compared to polyunsaturated substrates. Belongs to the amidase family.

Protein type: EC 3.5.1.99; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: integral to membrane

Molecular Function: carbon-nitrogen ligase activity, with glutamine as amido-N-donor; hydrolase activity

Biological Process: metabolic process