BMP-2 active protein

Human BMP-2

Cat. Num. AAA691663

• Gene Names: BMP2; BDA2; BMP2A
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: BMP-2; Human BMP-2; Recombinant Human Bone Morphogenetic Protein-2; BMP-2A; Bone morphogenetic protein 2A; BMP-2 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPF PLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVV LKNYQDMVVEGCGCR
• Sequence Length: 115

• Endotoxin Level: < 0.1ng per ug of BMP-2
• Biological Activity: Measured by the ability of BMP-2 to induce alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 0.3-0.8 ug/ml.
• Reconstitution: The lyophilized BMP-2 is best soluble in 50 mM acetic acid at a concentration of 0.1mg/ml but should be also soluble in most aqueous buffers when the pH is below 6.0.
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Reconstituted BMP-2 should be stored in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles!

Testing Data

Testing Data #2

Related Product Information for BMP-2 active protein

Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. The amino acid sequence of recombinant human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector. BMP-2 is a heparin binding protein.

Product Categories/Family for BMP-2 active protein

Cytokines & Growth Factors

References

1. Wozney et al., Science 242:1528, 1988 2. Ruppert et al., Eur J Biochem 237:295, 1996

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NM_001200.2
• UniProt Accession #: P12643
• Molecular Weight: 26.0 kDa
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: BMP2
• NCBI Official Synonym Symbols: BDA2; BMP2A
• NCBI Protein Information: bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: BMP-2-inducible protein kinase
• UniProt Gene Name: BMP2
• UniProt Synonym Gene Names: BMP2A; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Induces cartilage and bone formation.

Subunit structure: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN

By similarity. Ref.5

Subcellular location: Secreted.

Tissue specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Pharmaceutical use: Available under the name Infuse (Medtronic Sofamor Danek). Used for treating open tibial shaft fractures.

Sequence similarities: Belongs to the TGF-beta family.