BMP-2 active protein

Human BMP-2 Protein

Cat. Num. AAA9718680

• Gene Names: BMP2; BDA2; BMP2A; SSFSC
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BMP-2; Human BMP-2 Protein; Recombinant Human BMP-2 Protein (Bone morphogenetic protein 2) expressed in E. coli; BDA2; BDA2A; BMP2A; Bone morphogenetic protein 2; BMP2; BMP-2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 30 % CAN, 0.1 % TFA, pH 2.9.
• Sequence: Amino acid sequence derived from mature form of human BMP2 (NP_001191.1) (Gln283-Arg396) was expressed. The mature form sequences of human, mouse, rat, rhesus and canine BMP2 are identical.
• Sequence Length: 396

• Species: Human
• Protein Length: The recombinant human BMP2 monomer consists of 115 amino acids and has a predicted molecular mass of 13 kDa.
• Activity: Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2 mug/ml.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human BMP-2 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human BMP-2 protein.)

Related Product Information for BMP-2 active protein

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NP_001191.1
• UniProt Accession #: P12643
• Molecular Weight: 13 kDa
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: BMP2
• NCBI Official Synonym Symbols: BDA2; BMP2A; SSFSC
• NCBI Protein Information: bone morphogenetic protein 2
• Protein Family: BMP-2-inducible protein kinase

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]