Aldehyde Dehydrogenase, mitochondrial, 18-517aa Active Protein | Aldh3a2 active protein

Aldehyde Dehydrogenase, mitochondrial, 18-517aa, Recombinant, Human (ALDH2, ALDH-E2, ALDHI, ALDM)

Cat. Num. AAA636095

• Gene Names: Aldh3a2; Aldh4; FALDH
• Purity: Highly Purified; 90% (SDS-PAGE)
• Synonyms: Aldehyde Dehydrogenase; mitochondrial; 18-517aa; Recombinant; Human (ALDH2; ALDH-E2; ALDHI; ALDM); Aldh3a2 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, 1mM DTT, 1 mM EDTA, 10% glycerol.
• Sequence: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN; RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL; GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL; GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT; GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG; TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S

• Biological Activity: Specific activity is > 0.14 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols.
• Endotoxin Level: 1.0 EU/ug (LAL)
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(15% SDS-PAGE (3ug))

Related Product Information for Aldh3a2 active protein

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant

Product Categories/Family for Aldh3a2 active protein

Molecular Biology; MB-Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 205266
• NCBI GeneID: 65183
• UniProt Accession #: P30839
• Molecular Weight: 54.5kD
• NCBI Official Full Name: aldehyde dehydrogenase
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 3 family, member A2
• NCBI Official Symbol: Aldh3a2
• NCBI Official Synonym Symbols: Aldh4; FALDH
• NCBI Protein Information: fatty aldehyde dehydrogenase; msALDH; aldehyde dehydrogenase 4; aldehyde dehydrogenase 3A2; microsomal aldehyde dehydrogenase; aldehyde dehydrogenase family 3 member A2; alcohol dehydrogenase family 3, subfamily A2; aldehyde dehydrogenase family 3, subfamily A2
• UniProt Protein Name: Fatty aldehyde dehydrogenase
• Protein Family: Fatty aldehyde dehydrogenase
• UniProt Gene Name: Aldh3a2
• UniProt Synonym Gene Names: Aldh4; msALDH
• UniProt Entry Name: AL3A2_RAT

Uniprot Description

ALDH3A2: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; Membrane protein, integral; Endoplasmic reticulum; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - arginine and proline; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.3; Amino Acid Metabolism - histidine; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - lysine degradation; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Cellular Component: mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; peroxisome; nucleus; cytosol

Molecular Function: long-chain-alcohol oxidase activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; long-chain-aldehyde dehydrogenase activity

Biological Process: phytol metabolic process; response to reactive oxygen species; epidermis development; central nervous system development; formaldehyde metabolic process; aldehyde metabolic process; sesquiterpenoid metabolic process; peripheral nervous system development