Procathepsin K Active Protein | CATK active protein

Procathepsin K, rat recombinant

Cat. Num. AAA845004

• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Procathepsin K; rat recombinant; CTSK; CTSO; CTSO3; CATK active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 333

• Applicable Applications for CATK active protein: SDS-PAGE; HPLC
• Activity (Specifications/test method): >400 mU/mg
• Biological Activity: The specific activity is >400 mU/mg (1U = 1 umole/min/mg) as measured by Cathepsin K Activity Assay Kit
• Results: >400 mU/mg
• Unit Definition: 1 U = 1 umole/min/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CATK active protein

Background: Cathepsin K, a member of the papain cysteine proteinase family, is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is produced as an inactive proenzyme (35.4 kDa) that is converted to its mature active form (23.5 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.

Product Categories/Family for CATK active protein

Apoptosis; Non-Caspase Proteases; Cathepsin; Cathepsin EnzymesProteins and Enzymes; Proteases; CathepsinsProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 6978721
• NCBI GeneID: 25425
• NCBI Accession #: NP_037071.1
• NCBI GenBank Nucleotide #: NM_012939.1
• UniProt Accession #: P00786
• Molecular Weight: 35.4 kDa
• NCBI Official Full Name: pro-cathepsin H
• NCBI Official Synonym Full Names: cathepsin H
• NCBI Official Symbol: Ctsh
• NCBI Protein Information: pro-cathepsin H
• UniProt Protein Name: Pro-cathepsin H
• UniProt Gene Name: Ctsh
• UniProt Entry Name: CATH_RAT

NCBI Description

lysosomal protease that plays a role in protein metabolism [RGD, Feb 2006]

Uniprot Description

CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: Protease; Endoplasmic reticulum; EC 3.4.22.16

Cellular Component: extracellular space; lysosome; acrosome; axoneme; outer dense fiber; cytosol

Molecular Function: peptidase activity; protein self-association; HLA-A specific activating MHC class I receptor activity; cysteine-type endopeptidase activity; serine-type endopeptidase activity; endopeptidase activity; apoptotic protease activator activity; protein complex binding; caspase activator activity; kininogen binding; aminopeptidase activity; cysteine-type peptidase activity

Biological Process: caspase activation; adaptive immune response; response to retinoic acid; protein destabilization; apoptosis; membrane protein proteolysis; proteolysis; positive regulation of angiogenesis; positive regulation of cell proliferation; spermatogenesis; zymogen activation; surfactant homeostasis; metanephros development; immune response-regulating signal transduction; negative regulation of apoptosis; positive regulation of cell migration; T cell mediated cytotoxicity