Procathepsin K Active Protein | CATK active protein

Procathepsin K, human recombinant

Cat. Num. AAA844429

• Gene Names: CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Procathepsin K; human recombinant; CTSK; CTSO; CTSO3; CATK active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 329

• Applicable Applications for CATK active protein: SDS-PAGE; HPLC
• Biological Activity: The specific activity is >1000 mU/mg (1U = 1 umole/min/mg) as measured by Cathepsin K Activity Assay Kit
• Results: The specific activity is >1000 mU/mg (1U = 1 umole/min/mg) as measured by Cathepsin K Activity Assay Kit
• Unit Definition: 1U = 1 umole/min/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CATK active protein

Background: Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.

Product Categories/Family for CATK active protein

Apoptosis; Non-Caspase Proteases; Cathepsin; Cathepsin EnzymesProteins and Enzymes; Proteases; CathepsinsProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 4503151
• NCBI GeneID: 1513
• NCBI Accession #: NP_000387.1
• NCBI GenBank Nucleotide #: NM_000396.3
• UniProt Accession #: P43235; Q6FHS6
• Molecular Weight: 35.9 kDa
• NCBI Official Full Name: cathepsin K preproprotein
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Official Synonym Symbols: CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• NCBI Protein Information: cathepsin K
• UniProt Protein Name: Cathepsin K
• UniProt Gene Name: CTSK
• UniProt Synonym Gene Names: CTSO; CTSO2
• UniProt Entry Name: CATK_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Uniprot Description

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.38; Protease

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; extracellular region

Molecular Function: collagen binding; proteoglycan binding; protein binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; intramembranous ossification; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption

Disease: Pycnodysostosis