Western Blot (WB)
(Western blot analysis of GAL3ST1 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). GAL3ST1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse GAL3ST1 Polyclonal Antibody | anti-GAL3ST1 antibody
GAL3ST1 Antibody (Center)
Gene Names
GAL3ST1; CST
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GAL3ST1; Polyclonal Antibody; GAL3ST1 Antibody (Center); Galactosylceramide sulfotransferase; GalCer sulfotransferase; 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; Cerebroside sulfotransferase; CST; anti-GAL3ST1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GAL3ST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the Central region of human GAL3ST1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
88-116
Sequence Length
423
Applicable Applications for anti-GAL3ST1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of GAL3ST1 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). GAL3ST1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GAL3ST1 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). GAL3ST1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GAL3ST1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GAL3ST1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GAL3ST1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GAL3ST1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GAL3ST1 antibody
Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. GAL3ST1 is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma.
Product Categories/Family for anti-GAL3ST1 antibody
References
Siegrist,H.P., et.al., Biochim. Biophys. Acta 489 (1), 58-63 (1977)
Stein,C., et.al., J. Biol. Chem. 264 (2), 1252-1259 (1989)
Stein,C., et.al., J. Biol. Chem. 264 (2), 1252-1259 (1989)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48764
NCBI Official Full Name
galactosylceramide sulfotransferase
NCBI Official Synonym Full Names
galactose-3-O-sulfotransferase 1
NCBI Official Symbol
GAL3ST1
NCBI Official Synonym Symbols
CST
NCBI Protein Information
galactosylceramide sulfotransferase
UniProt Protein Name
Galactosylceramide sulfotransferase
Protein Family
UniProt Gene Name
GAL3ST1
UniProt Synonym Gene Names
CST; GalCer sulfotransferase
UniProt Entry Name
G3ST1_HUMAN
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Product Notes
The GAL3ST1 gal3st1 (Catalog #AAA9207417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 88-116. The GAL3ST1 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GAL3ST1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the GAL3ST1 gal3st1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAL3ST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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